Hypercholesterolaemia affects Sertoli and Leydig cells secretory functions, impairs steroid hormones biosynthesis, and disrupts the hypothalamic-pituitary-gonadal axis. The use of honey in previous studies resulted in an improvement of male reproductive hormonal disturbances. This study aimed to investigate the protective effects of Trihoney on hypercholesterolaemia-induced male reproductive hormonal changes in male rabbits and compare its effects with atorvastatin. Materials and methods: Forty-eight male New Zealand white rabbits were assigned into 6 groups as follows; Control: commercial pellet; CH: commercial pellet with 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet with 0.3 g/kg/day Trihoney; DH2: 1% cholesterol diet with 0.6 g/kg/day Trihoney and DAt: 1% cholesterol diet with 2mg/kg/day atorvastatin. After 12 weeks, the rabbits were sacrificed and blood samples were collected for analysis of hormones and pro-inflammatory cytokines and calculation of HOMA-IR. The testes were homogenized for intra-testicular testosterone measurement. Results:Serum testosterone reduced significantly in HCD (p