Background: Potassium (K+) is the essential micronutrient and major intracellular fluid cation which involves in various cellular metabolism activities, maintaining fluid and electrolyte balance. Measurement of blood concentration in a medical laboratory has often encountered disturbances such as hemolysis, which may lead to the elevation in measurement and affects the medical diagnosis and treatment of the patient, conceivably fatal. Hemolysis can be decided using hemolysis index (H-index) through automation. Methods: In this study, H-index and concentration of fifty hospitalized patients (n=50) hemolysed blood samples were measured and correlated. Freezing-and-thaw method was used to hemolyse the blood samples. Different concentrations were diluted and analyzed using COBAS 8000 biochemistry analyser. Data were collected and analyzed using SPSS version 25. Results: Our findings showed significant mean differences, 0.001 (p ≤ 0.05) and strong positive linear relationship between two variables (H-index and ) (r=0.764, p ≤ 0.05). By applying calculated linear equation [y = 0.0048x + 5.146, = 0.5838], critical value of 6.0 mmol/l gives H-index of 178, H-index above 178 is suggested to be critical. Discussion and Conclusion: Concentration increases in proportion to H-index. A greater degree of hemolysis causes more ions to be released into extracellular fluid, respectively. In conclusion, when H-index less than 178 in measurement and there is no analytical significance bias generated, the result is acceptable, whilst H-index with analyte variation between clinically significant bias range can be released with a comment regarding the potential of data alteration. Meanwhile, result with H-index exceeding the cut-offs should be suppressed and recollection of sample is required.
ABO blood grouping is an important antigenic blood typing tools in blood transfusion and organ transplants. Mismatching of blood during transfusion would lead to undesired transfusion reactions. Due to rare occurrence of rare blood group such as A2 subtype, regular blood grouping technique would have missed the identification of blood group. Objectives: In this study, the identification of A2 subgroup using routine serological technique was validated via DNA sequencing technique. Materials and Methods: A total of 656 students participated in this study consist of Malay (87.0 %), Chinese (0.4 %), Indian (11.4 %) and others ethnic group (0.9%) respectively. Monoclonal antisera A, B, AB, D, A1 lectin and H lectin were used to identify the antigen on red blood cells. DNA sequence analysis was applied to examine single nucleotide polymorphisms (SNPs) at position 467 (substitution of C>T) and 1061 (deletion of C) on coding region of ABO gene. Results: Our findings showed of 656 blood samples, 256 (39.0%) were blood group O, 190 (29.0%) were blood group B, 179 (27.3%) were blood group A and 31(4.7%) were blood group AB. The frequency of A1 subgroup is 177 (99.0%) and A2 subgroup is 2 (1.0%). From 179 A blood group, only 2 samples showed negative reaction towards anti-A1 lectin. DNA sequence analysis revealed the SNPs at nucleotide 1061 position in sample 2, however this mutation was absence in sample 1, suggesting presence of another mutation that may result in the A2 phenotype. Conclusion: The current study reported the absence of 1061C deletion in A2 blood group sample among Malaysian population.