This study investigated the jumping performance and lower limbs kinematics among boys with Down syndrome. The participants (n = 23) were required to perform standing broad jump test and their jumping performance was evaluated using Motor Skills Inventory for locomotor skills analysis. In addition, the jumping performance was also recorded for lower limb kinematic analysis using 2-Dimensional video recording tools. Results revealed that 91.3% of the participants scored ‘Poor’ values of standing broad jump distances. Based on the Motor Skills Inventory analysis, five participants were grouped as ‘Rudimentary’, nine participants were ‘Functional’ and the other nine participants were ‘Mature’ level of motor development. Based on these three groups, further analysis was conducted on the lower limbs kinematics during jumping performance (three phases: take off, jump peak height and landing). Finding showed that there were no significant differences on lower limb kinematics between the groups during these three phases of jumping. Proper intervention strategies are needed in order to improve the jumping skills among children with Down syndrome.