Mangosteen is a native fruit from Southeast Asia. It is rich in phenolic compounds like xanthones, anthocyanins and phenolic acids and also a good source of fibre and minerals. The present study aim to investigate the effects of mangosteen aril supplementation on the histopathological changes of liver and kidney in rats fed with high fat diet. Forty male Sprague Dawley rats were divided into five groups (n=8), which consisted of normal control group (NC), obese control group (OC), obese supplemented with 200 mg/kg mangosteen group (M200), obese supplemented with 400 mg/kg mangosteen group (M400) and obese supplemented with 600 mg/kg mangosteen group (M600). At the end of seven weeks, obese groups supplemented with mangosteen aril were force feed to correspond mangosteen dosage while the control groups were force feed with distilled water as placebo. At the end of seven weeks of supplementation period, all rats were sacrificed and liver and kidney were collected. All data were analyzed using one way ANOVA and the differences between groups were considered significant at p < 0.05. Results showed that supplementation of mangosteen aril in obese rats able to ameliorate the abnormalities in their liver and kidney tissue caused by high fat diet.