The 13-item Body Acceptance by Others Scale-2 (BAOS-2) measures generalised perceptions of body acceptance by others. Here, we examined the psychometric properties of a novel Italian translation of the BAOS-2. Studies 1 (N = 401) and 2 (N = 393) supported a unidimensional model of the Italian BAOS-2 with all 13 items retained through exploratory and confirmatory factor analysis, respectively. Study 2 also supported measurement invariance of the Italian BAOS-2 across gender identity, with the gender difference in observed scores not reaching significance. In Study 3 (N = 365), we supported the construct validity of the Italian BAOS-2. Specifically, we found evidence of convergent validity (significant associations with indices of positive body image), criterion-related validity (significant associations with pressure to conform to appearance ideals and psychological well-being), and incremental validity (body acceptance predicted body appreciation over-and-above appearance-related pressure). These results suggest that the psychometric properties of the Italian BAOS-2 are robust and that this instrument can be confidently utilised in future research with Italian adults.