The PadiU Putra rice line is a blast-resistant and high-yield rice line with high potential. The application of topdressing and the foliar applied method of silicon (Si) treatments could strengthen the culm to resist breakage and ultimately increase yield production. Treatments which consisted of a control, a Si topdressing, and a Si foliar applied were arranged in a randomised complete block design. At 55 days after transplanting (DAT), the foliar applied Si treatments had 59% higher dry matter partitioning to the roots. Meanwhile, at 75 DAT, both Si foliar applied and topdressing method showed increased assimilate partitioning into the culm sheath by 29% and 49%, respectively. Dark green and light yellowish colours were obtained in both Si treatments using UAV, indicating similar results to physiological responses. Remarkably, Si foliar applied treatments enhanced the diameter and width of the outer and inner layers of the diameter of vascular bundles at 75 DAT by 58, 181, and 80%, respectively. The yield production of rice increased by 53% in the Si foliar applied, compared to the control, and produced a 1.63 benefit-cost ratio.