Phosphatases are extracellular enzymes that catalyze the hydrolysis of phospho-ester bonds inorganic phosphate-containing substrates and release inorganic phosphate in the arrangement of orthophosphates that able to be consumed by organisms. In the current years, there has been a major escalation in the number of studies and publications focusing on alkaline phosphatases (ALPs). This expansion reveals more complexity of the functions of this enzyme. This review highlights multiple roles played particularly on ALP in relation to human health, aquaculture, bacteria, and mammals. Continuous research on this multifaceted enzyme will undeniably offer better insight into understanding the overall function in every living organism.