Black spot disease is a significant worldwide disease on the rose plant. Due to this infection, the leaves become yellow and eventually fall off. The occurrence of this disease has become a major problem, especially in landscape purpose. Therefore, this research was conducted to isolate fungal species from black spot disease in rose and identify using morphological characteristics. Then, all the isolates were tested for pathogenicity to confirm Koch’s postulates. In this study, four fungal isolates have been successfully isolated from black spot disease in rose namely Rhizoctonia sp. (one isolate), Colletotrichum sp. (two isolates) and Penicillium sp. (one isolate). Based on pathogenicity test result using potato dextrose agar (PDA) plug technique, fungus UMTT27R (Penicillium sp.) showed highly pathogenic on rose’s leaves with disease severity (DS) = 88.89% followed by UMTT13R (Colletotrichum sp.) with DS=72.22%, UMTT21R (Colletotrichum sp.) with DS=66.67% and UMTT4R (Rhizoctonia sp.) with DS=61.11%. Correct identification of fungal pathogens is very important to strategize a proper method to control the black spot disease in rose cultivation.
Many chilli producers in Malaysia are facing a huge problem of anthracnose disease that infect the chilli fruits either at pre- or post-harvest stage. Due to this infection, the chilli fruits become rotten and could be colonized by grey conidial masses which is unfit for human consumption and cannot be sold. This study was aimed to isolate the fungal species associated with anthracnose symptoms collected from several mini-markets near Gong Badak, Terengganu. Pathogenicity test was conducted for all these isolates to evaluate their pathogenic level. Based on the results, five fungal isolates were successfully isolated and morphologically identified as Colletotrichum spp. All these isolates were labelled as UMTT46C, UMTT48C, UMTT50C, UMTT52C and UMTT54C. From these, fungal isolate (UMTT54C) showed the highest pathogenic level with disease severity (DS), 68.9%. Other Colletotrichum spp. recorded percentage of DS ranged from 53.3% to 60%. Identification of fungal isolates and their pathogenic level from the infected chilli is very important to assist in control strategy which can further provide a good protection measure for many chilli producers.