Mosquito-borne diseases have wreaked havoc on human health, with consequences dramatically increasing in recent years. The incidence of mosquito-borne diseases is closely linked to the locations that are chosen for urban development. The aim of this study was to provide characteristics of mosquito breeding sites in northern and southern China and to document the most important arbovirus vectors found in the study area, the evidence generated here is critical for early prevention and control inter ventions. This research involved a random selection of various sites across four provinces, spanning both the northern and southern regions of China. The dwellings and accessible water storage containers in these sites were investigated to detect the presence of immature mosquitoes. Samples were then collected, mosquitoes were nurtured to adulthood, and the species that were present were identified. A total of 1 249 samples were collected during this survey of the mosquito breeding sites. A total of 80 samples were processed using the Chelex method to extract mosquito DNA from all the samples. The ITS2 gene fragment was then amplified by PCR and sequenced. A subsequent BLAST comparison allowed the identification of the mosquito species, and MEGA11 software was used for phylogenetic analysis. The results showed that there were four species of mosquitoes, including Aedes albopictus, Culex quinquefasciatus, Lutzia fuscanus and Armigeres subalbatus. The primary mosquito breeding grounds in the four provinces of China consisted of storm drains, discarded containers, garbage bins, and areas with standing water. Still-water environments, such as rice fields were the primary breeding locations in the southern cities. In contrast, in the northern regions, most breeding occurred at construction sites, and in similar water-prone areas. The most prevalent mosquitoes in the four provinces of China were of the genus Aedes, with a significant number originating from Fujian Province, China. This information sheds light on the migration patterns of mosquitoes and significantly enhances community-based protection measures and mobilization efforts.
The aim of this study was to determine whether primary repair for intraoperative injury of the medial collateral ligament (MCL) can achieve satisfactory clinical results when compared to the clinical results of patients with no MCL injury. Simultaneously, we sought to determine the differences between 2 methods of primary repair (anchor suture and staple) in terms of their clinical outcomes.In our institute, 3897 total-knee arthroplasties (TKAs) were performed between 2003 and 2014. Sixty-five patients who suffered an MCL injury during the TKA procedure and in whom the injury was repaired with a suture anchor or staple (suture anchor: 36 vs staple: 29) were studied. A matched group of 65 patients without an MCL injury was selected to serve as the control group. Subjective feelings of instability and functional outcomes were assessed using the knee society (KS) score and the Western Ontario & McMaster Universities Osteoarthritis Index (WOMAC). Objective stability was evaluated by the measurement of opening angles in extension and at 30° of knee flexion on valgus stress radiographs. The clinical outcomes and stability results were compared between the suture anchor and staple methods.The KS and WOMAC scores in patients who received primary repair of MCL injury during TKA improved from 50.6 ± 13.1 to 87.3 ± 7.3 (P
The Qinghai-Tibet Plateau endemic Chinese mountain cat has a controversial taxonomic status, whether it is a true species or a wildcat (Felis silvestris) subspecies and whether it has contributed to cat (F. s. catus) domestication in East Asia. Here, we sampled F. silvestris lineages across China and sequenced 51 nuclear genomes, 55 mitogenomes, and multilocus regions from 270 modern or museum specimens. Genome-wide analyses classified the Chinese mountain cat as a wildcat conspecific F. s. bieti, which was not involved in cat domestication of China, thus supporting a single domestication origin arising from the African wildcat (F. s. lybica). A complex hybridization scenario including ancient introgression from the Asiatic wildcat (F. s. ornata) to F. s. bieti, and contemporary gene flow between F. s. bieti and sympatric domestic cats that are likely recent Plateau arrivals, raises the prospect of disrupted wildcat genetic integrity, an issue with profound conservation implications.