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Host-Parasite Interactions and Integrated Management Strategies for Ecytonucleospora Hepatopenaei Infection in Shrimp

Haridevamuthu B, Sudhakaran G, Rajagopal R, Alfarhan A, Arshad A, Arockiaraj J

Acta Parasitol, 2025 Mar 06;70(2):67.
PMID: 40050501 DOI: 10.1007/s11686-025-01007-0

BACKGROUND: Ecytonucleospora hepatopenaei (EHP) is a major parasitic pathogen in shrimp causing hepatopancreatic microsporidiosis, which leads to significant growth retardation and global economic losses. This pathogen employs various immune evasion strategies that complicate treatment and management.
PURPOSE: This review examines the complex host-parasite interactions, focusing on the immune evasion mechanisms used by EHP. The study explores how EHP manipulates host immune pathways, including NF-κB, JAK/STAT, Toll, and IMD, to suppress immune responses, inhibit antimicrobial peptide production, and avoid detection, thus ensuring its persistence in the host.
METHODS: The authors reviewed recent research from databases like PubMed, Scopus, and Web of Science, including studies up to 2024. The keywords Ecytonucleospora hepatopenaei, immune evasion, EHP treatment, and associated words with topics were used in this search.
RESULTS: EHP induces oxidative stress, which weakens the host immune system while simultaneously upregulating antioxidant responses to favor its survival. The parasite also alters the gut microbiota and disrupts key cellular processes, such as cell cycle regulation, further enhancing its ability to sustain infection.
CONCLUSION: This review highlights the need for integrated management strategies, including disease-resistant breeding, microbiota modulation, and advanced diagnostics, to combat EHP. By providing an overview of EHP's immune evasion tactics, this study aims to advance knowledge in the field and support efforts to improve shrimp health and aquaculture sustainability.

Matched MeSH terms: Enterocytozoon/genetics
Molecular detection of porcine Enterocytozoon bieneusi infection in Peninsular Malaysia and epidemiological risk factors associated with potentially zoonotic genotypes

Ruviniyia K, Abdullah DA, Sumita S, Lim YAL, Ooi PT, Sharma RSK

Parasitol Res, 2020 May;119(5):1663-1674.
PMID: 32219552 DOI: 10.1007/s00436-020-06648-w

Enterocytozoon bieneusi is an emerging opportunistic pathogen infecting humans, and both domestic and wild pigs are known to harbour zoonotic genotypes. There remains a paucity of information on the prevalence and epidemiology of this enteropathogen in Southeast Asia. The present study was undertaken to determine the molecular prevalence and risk factors associated with E. bieneusi infection among commercially farmed pigs in Malaysia. Faecal samples were collected from 450 pigs from 15 different farms and subjected to nested PCR amplification of the ribosomal internal transcribed spacer (ITS) gene of E. bieneusi. Phylogenetic analysis involved 28 nucleotide sequences of the ITS region of E. bieneusi. An interviewer-administered questionnaire provided information on the animal hosts, farm management systems and environmental factors and was statistically analysed to determine the risk factors for infection. The prevalence of E. bieneusi infection was relatively high (40.7%). The highest prevalence (51.3%) was recorded among the piglets, while the adults showed the lowest level of infection (31.3%). Multivariate analysis indicated that age of the pigs, distance of the farm from human settlement and farm management system were significant risk factors of infection. Three genotypes (EbpA, EbpC and Henan-III) detected among the pigs are potentially zoonotic. The high prevalence of E. bieneusi among locally reared pigs, the presence of zoonotic genotypes and the spatial distribution of pig farms and human settlements warrant further investigation on the possibility of zoonotic transmission.

Matched MeSH terms: Enterocytozoon/genetics
Molecular detection and species identification of Enterocytozoon bieneusi isolated from immunocompetent Orang Asli in Malaysia

Ashikin A, Al-Mekhlafi HM, Moktar N, Anuar TS

Parasitol Int, 2017 Apr;66(2):163-165.
PMID: 28115231 DOI: 10.1016/j.parint.2017.01.013

Most studies of opportunistic infections focus on immunocompromised patients. However, there is a lack of information on microsporidiosis in healthy people (immunocompetent) worldwide. This study aimed to detect and identify microsporidia species in immunocompetent Orang Asli living in Pahang, Malaysia. Orang Asli is a collective term for a group of indigenous people that usually reside in the interior regions of Peninsular Malaysia. They comprise about 0.7% of the total population in Malaysia and 76% of them lived below the poverty line i.e., poor housing conditions with the lack of access to safe drinking water and adequate sanitation, contaminated environment, high illiteracy rate and unhygienic practices by these people. Stool samples were collected from 209 Orang Asli and analyzed for detecting the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis by polymerase chain reaction assay targeting small subunit ribosomal RNA gene. E. bieneusi was detected in 8 individuals (3.83%). This infection was commonly found in males than females (5.2% vs. 2.7%). All infected Orang Asli were adults, with a mean age of 44years. Diarrhea and other gastrointestinal symptoms were reported in one case (12.5%) among individuals infected with this species. These findings clearly show that exposure to E. bieneusi may actually be common than reported. The accurate detection and identification of microsporidian species by molecular technique will improve therapy, clinical manifestations and prognosis of this infection, as no antiparasitic therapy has been approved for E. bieneusi. It is hoped that these findings will allow the formulation of better health management and disease prevention advisories, and improvement in the standards of health in similar communities.

Matched MeSH terms: Enterocytozoon/genetics*
Fulltext Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia

Hassan NA, Lim YAL, Mahmud R, Mohd-Shaharuddin N, Wan Sulaiman WY, Ngui R

Am J Trop Med Hyg, 2018 Dec;99(6):1562-1566.
PMID: 30382015 DOI: 10.4269/ajtmh.17-0901

Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out to identify microsporidian species in immunocompromised patients. From March 2016 to March 2017, a total of 289 archived stool samples were examined microscopically for microsporidian spores using Gram-chromotrope Kinyoun (GCK) stain. Positive stool samples by microscopy were subjected to polymerase chain reaction and sequencing for species identification. Based on microscopy examination, the overall prevalence of microsporidian infections was 32.2% (93/289; 95% CI = 27.1-37.8). Of these stool samples, 45 samples were successfully amplified and confirmed as Enterocytozoon bieneusi. No Encephalitozoon intestinalis was detected. Accurate identification of species might help clinicians to decide appropriate management strategies as dissemination risks and treatment response vary for different species, hence improving the management of microsporidian infections.

Matched MeSH terms: Enterocytozoon/genetics*