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On optimizing the blocking step of indirect enzyme-linked immunosorbent assay for Epstein-Barr virus serology
Lim CS, Krishnan G, Sam CK, Ng CC
Clin Chim Acta
, 2013 Jan 16;415:158-61.
PMID: 23043757
DOI:
10.1016/j.cca.2012.08.031
Abstract
Because blocking agent occupies most binding surface of a solid phase, its ability to prevent nonspecific binding determines the signal-to-noise ratio (SNR) and reliability of an enzyme-linked immunosorbent assay (ELISA).
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Ficoll/chemistry
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