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  1. Wong WL, Tan WB, Lim LH
    J Helminthol, 2006 Mar;80(1):87-90.
    PMID: 16469180
    Hard structures of helminths have often been used for taxonomic identification but are usually not clearly defined when treated with conventional methods such as ammonium picrate-glycerin for monogeneans and glycerin for nematodes. The present study reports a rapid and simple technique to better resolve the hard parts of selected monogeneans and nematodes using 5-10% alkaline sodium dodecyl sulphate (SDS). In comparison with established methods, SDS-treated worms become more transparent. In monogeneans treated with SDS, clear details of the hooks, hook filaments, anchors, bars and the sclerotized copulatory organs could be observed. In SDS-treated nematodes, spicules and ornamentations of the buccal capsules could be clearly seen.
    Matched MeSH terms: Microscopy, Phase-Contrast/methods
  2. Ali- Saeed R, Alabsi AM, Ideris A, Omar AR, Yusoff K, Ali AM
    Asian Pac J Cancer Prev, 2019 Mar 26;20(3):757-765.
    PMID: 30909682
    Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest
    of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells.
    Methods: In this investigation, the proliferation of brain tumor cell line, glioblastoma multiform (DBTRG.05MG)
    induced by NDV strain AF2240 was evaluated in-vitro, by using MTT proliferation assay. Furthermore, Cytological
    observations were studied using fluorescence microscopy and transmission electron microscopy, DNA laddering in
    agarose gel electrophoresis assay used to detect the mode of cell death and analysis of the cellular DNA content by
    flowcytometery. Results: MTT proliferation assay, Cytological observations using fluorescence microscopy and
    transmission electron microscopy show the anti-proliferation effect and apoptogenic features of NDV on DBTRG.05MG.
    Furthermore, analysis of the cellular DNA content showed that there was a loss of treated cells in all cell cycle phases
    (G1, S and G2/M) accompanied with increasing in sub-G1 region (apoptosis peak). Conclusion: It could be concluded
    that NDV strain AF2240 is a potent antitumor agent that induce apoptosis and its cytotoxicity increasing while increasing
    of time and virus titer.
    Matched MeSH terms: Microscopy, Phase-Contrast/methods*
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