Affiliations 

  • 1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia. theamsoon@usm.my
  • 2 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia
Methods Mol Biol, 2023;2702:275-290.
PMID: 37679625 DOI: 10.1007/978-1-0716-3381-6_14

Abstract

Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually screened via repetitive rounds of antigen-guided selection and phage amplification. The main advantage of this approach lies in the physical linkage between phenotype and genotype. This feature allows the isolation of single unique clones from a panning campaign consisting of a highly diverse population of clones. Due to the high-throughput nature of this technique, different approaches have been developed to assist phage display selections. One of which involves utilizing a streptavidin-coated solid-phase extraction (SPE) tip that is mounted to an electronically controlled motorized multichannel pipette. In this chapter, we will entail the procedures involved in the adaptation of a commercial SPE tip (MSIA™ streptavidin D.A.R.T's®) as the solid phase. This protocol is an updated version of a previous protocol with some minor refinements.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.