Box-Behnken Design and Molecular Docking Assisted Quenching Spectrofluorimetric Method for the Quantitation of Citalopram HBr in Commercial Dosage Forms

Azmi SNH 1 , Rawahi WANA 2 , Nabhani ZSZA 2 , Al Farsi AAAA 2 , Ahmed QU 3 , Alam M 4 Show all authors , Mia MAR 5 , Rahman N 6

Affiliations 

  • 1 Applied Sciences Department, College of Applied Sciences and Pharmacy, University of Technology and Applied Sciences-Muscat, Al Khuwair 133, P. O. Box 74, Muscat, Sultanate of Oman. syed.najmul@utas.edu.om
  • 2 Applied Sciences Department, College of Applied Sciences and Pharmacy, University of Technology and Applied Sciences-Muscat, Al Khuwair 133, P. O. Box 74, Muscat, Sultanate of Oman
  • 3 Drug Discovery and Synthetic Chemistry Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Pahang Darul Makmur, Kuantan, 25200, Malaysia
  • 4 Division of Smart Safety Engineering, Dongguk University Wise, 123 Dongdae-ro, Gyeongju-si, 780714, Republic of Korea
  • 5 Cooperative Research, Extension, and Education Services (CREES), Northern Marianas College, P.O. Box 501250, Saipan, MP, 96950, USA
  • 6 Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India
J Fluoresc, 2025 Feb 18.
PMID: 39964649 DOI: 10.1007/s10895-025-04162-9

Abstract

A simple, sensitive and accurate spectrofluorimetric method was presented for the determination of citalopram hydrobromide (CTM) in tablets. The method was based on the quenching of bovine serum albumin (BSA) fluorescence with CTM at pH 7.4. The fluorescence intensities were measured at 342 nm after excitation at 279 nm. Various factors affecting the quenching of BSA fluorescence were optimized by response surface methodology (RSM) through Box-Behnken design (BBD. The plot of F0/F versus CTM concentration was linear in the concentration range of 10-100 µg mL-1. The limit of detection (LOD) and limit of quantitation (LOQ) were 2.08 and 6.30 µg mL-1, respectively The molar combining ratio between CTM and BSA was 1:1. The complementary modified green analytical procedure index (ComplexMoGAPI) was 90%. The effect interference of common excipients found in tablets was investigated. Percent recoveries of CTM was ranged from 99.92 to 100.27% in tablets.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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