Metabolomic and physiological analyses of two picochlorophytes from distinct oceanic latitudes under future ocean acidification and warming

Phytoplankton are cosmopolitan marine photosynthetic organisms that are vital to biogeochemical cycles and marine ecosystems. The current rise in atmospheric CO2 and surface ocean temperatures are poised to disrupt the ecological niches of phytoplankton. Picochlorophytes, a broad taxon of small green eukaryotic phytoplankton, have been shown to perform well under future rising oceanic CO2 and temperature scenarios. This study investigates the acclimation responses of cosmopolitan picochlorophytes from the Chlorella-lineage under high CO2 (1000 p.p.m.) and a rise of 4 °C (8 °C - polar picochlorophyte; 32 °C, tropical picochlorophyte). In order to determine how the future ocean warming and acidification might affect picochlorophytes, a polar strain of Chlorella and a tropical Parachlorella were selected, and their physiology and GCMS-based metabolomics were investigated. Growth rate and cellular dimensions (diameter, volume, and surface area) of Chlorella significantly increased in all environmental future scenarios compared to Parachlorella. Photosynthetic parameters of the picochlorophytes studied showed acclimation, with high temperature and high CO2 triggering the adaptation of Fv/Fm, NPQmax, and Ek of Chlorella and Parachlorella, respectively. High CO2 induced the most changes in the Chlorella metabolome, altering the levels of metabolites related to amino acids and their derivatives, glutathione production, carbohydrates, and photochemical quenching. Combined high CO2/temperature altered Parachlorella's metabolome, though with a small number of biomarkers detected. This study provided evidence to support the hypothesis that picochlorophytes could thrive in a more acidified and warmer ocean.