Betacyanins are water soluble pigment commonly detected in plants. The most studied betacyanins pigment is betanin group. Betanin has been shown to inhibit lipid peroxidation and heme decomposition, suggesting that these pigments may provide protection against certain oxidative stress-related disorders. The aim of this study was to investigate the changes in hepatic and heart structure in high-carbohydrate, high-fat diet-induced obese rats following supplementation of betanin. A total of 48 male Wistar rats were divided into 4 groups: corn-starch group (C), corn-starch + betanin group (CB), high-carbohydrate, high fat group (H) and high-carbohydrate, high fat + betanin (HB).The intervention with 50 mg betanin/kg of food was started for 8 weeks after 8 weeks initiation of the diet. Liver and cardiac structures were defined by histology. Results showed that betanin supplemented rats showed normalized inflammatory state with no change in collagen deposition in the heart but did not normalize the macrovesicular steatosis in the liver. The effects of betanin supplementation were more prominent in corn starch fed rats suggesting its potential in preventing fat accumulation possibly by its potent scavenging ability from the pigment, but not reversing the abnormalities related to obesity.