Workplace bullying has been recognized as a serious issue that may affect psychological stress symptoms of employees and jeopardize organization’s productivity especially in the healthcare sector. This study aims to identify the influence of personality traits on workplace bullying among registered nurses in a public hospital. A total of 340 registered nurses were involved in this study using purposive sampling. Two sets of questionnaires were used in this study, namely International Personality Item Pool (IPIP) for measuring personality and instrument of Negative Acts Questionnaire-Revised (NAQR) for measuring workplace bullying. The data obtained were analyzed using Statistical Package for Social Sciences (SPSS) version 23. The multiple linear regression analysis indicates that only personality trait of conscientiousness negatively affects work-related bullying, person-related bullying, and physically intimidating bullying. This finding clarifies that low conscientiousness requires more attention from organizations in order to reduce bullying at work. Therefore, this factor may serve as a predictor to evaluate the suitability of future nurses in personnel decision making