In Malaysia, women had breast cancer always reported at their late stage. One of the causes is due to the delay in seeking medical attention. Poor knowledge about the breast cancer is one of the factors that cause the delay. Aim: This study was designed to assess the knowledge on breast cancer symptoms and risk factors, screening method, and practice among participants. Methodology: This was a cross-sectional study done from 1st of March till 15th of March 2016 involving 89 nursing students from School of Nursing Science, Medical Faculty, University Sultan Zainal Abidin (UniSZA). Data analysis was carried out using Statistical Package for the Social Sciences (SPSS) Version 21. Results: Eighty-nine participants responded. Majority of the students (>80.0%) knew the symptoms of breast cancer and common method of screening. Less than 50.0% knew the high risk factor for developing breast carcinoma. Less than 50.0% carried out the breast self-examination. Conclusion: Students had excellent knowledge on symptoms of breast carcinoma and its screening method. Majority (>50%) of the students were still not clear about high risk factors group. Lack of breast self-examination practice among students was noted.