Affiliations 

  • 1 Department of Biological Sciences, Sunway University, Bandar Sunway, Selangor, 47500, Malaysia
  • 2 Department of Biological Sciences, Sunway University, Bandar Sunway, Selangor, 47500, Malaysia. Electronic address: jacttyc@sunway.edu.my
Exp Parasitol, 2020 Feb 19;211:107862.
PMID: 32087220 DOI: 10.1016/j.exppara.2020.107862

Abstract

Chryseobacterium indologenes and Chryseobacterium gleum are Gram negative environmental bacteria that have been frequently reported to implicate in fatal nosocomial infections, such as bacteraemia and ventilator-associated pneumonia in immunocompromised individuals in the past decades. The interaction between Chryseobacterium spp. and Acanthamoeba castellanii, a free-living amoeba ubiquitous in the environment, has not been explored previously. In this study, C. indologenes and C. gleum were co-cultured with A. castellanii trophozoites and their interactions were evaluated. Our results showed that when co-cultured with A. castellanii, bacterial numbers of C. indologenes and C. gleum increased significantly (p  0.05). Interestingly, the two Chryseobacterium spp. associated, invaded and/or taken up by A. castellanii at significantly higher rates than Escherichia coli K1, a neuropathogenic bacterial strain known to interact and replicate intracellularly in A. castellanii (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.