Affiliations 

  • 1 Faculty of Medicine, Lincoln University College, Petaling Jaya, Selangor D. E., Malaysia; Medical Faculty, Baiturrahmah University Padang, West Sumatera. dessyabdullah@fk.unbrah.ac.id
  • 2 Lincoln University College, Wisma Lincoln, Petaling Jaya, Selangor D. E.. sandeeppoddar@lincoln.edu.my
  • 3 Lincoln University College, Wisma Lincoln, Petaling Jaya, Selangor D. E.. ramesprasath@lincoln.edu.my
  • 4 Faculty of Animal Science, University of Andalas, Padang, West Sumatera. purwati17@ansci.unand.ac.id
  • 5 Faculty of Medicine, Lincoln University College, Petaling Jaya, Selangor D. E., Malaysia; Medical Faculty, Baiturrahmah University Padang, West Sumatera. nadiapurnama.op@gmail.com
  • 6 Doctoral Program, Faculty of Animal Science, University of Andalas, Padang. yudhaendra.pratama@gmail.com
J Public Health Res, 2021 Nov 24;10(s2).
PMID: 34818881 DOI: 10.4081/jphr.2021.2508

Abstract

BACKGROUND: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB), which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra.

DESIGN AND METHODS: A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16S rRNA gene.

RESULTS: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zone area of 23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp.

CONCLUSIONS: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.