The genomics and genetic information of Malaysian rice (Oryza sativa L.) is currently limited. It was necessary to conduct genome resequencing of these rice accessions exhibiting different responses to salinity stress. The sequencing was carried out using the Illumina NovaSeq X platform with 30× sequencing coverage to pinpoint variants between salinity tolerant and sensitive rice accessions. The discovery of single nucleotide polymorphisms (SNPs) is crucial for the development of DNA markers associated with salinity tolerance traits. The genome sequence data (FASTQ format) for these accessions have been deposited to the European Nucleotide Archive (ENA) database under the accession number PRJEB71716.
In Malaysia, rice mutant varieties that are genetically altered to confer resistance against blast disease have been substantially developed through mutational breeding program. However, due to the limited accessible information on the mutant lines, mutant gene variants corresponding to the disease resistance and other useful agronomic traits are yet to be exploited. Here, we conducted whole genome re-sequencing of blast resistance with kernel elongation traits in mutant line, Mahsuri Mutant (87,639,446 bp raw reads), and its parental line, Mahsuri (85,156,783 bp raw reads) using Illumina Novaseq 6000 sequencing platform with 30x sequencing coverage. The generated genome sequences are aimed to facilitate the discovery of causal mutation and single nucleotide polymorphisms (SNPs) related to the intended traits. The identified SNPs can be further employed to develop allele-specific SNP molecular markers to locate the mutant gene regions. The NGS data obtained (FASTQ format) of the parental and mutant lines have been deposited in the National Center for Biotechnology Information (NCBI) database under sequence read archive (SRA) xwith accession numbers SRR24388814 (Mahsuri) and SRR22952097 (Mahsuri Mutant) respectively.