Hypercholesterolaemia affects Sertoli and Leydig cells secretory functions, impairs steroid hormones biosynthesis, and disrupts the hypothalamic-pituitary-gonadal axis. The use of honey in previous studies resulted in an improvement of male reproductive hormonal disturbances. This study aimed to investigate the protective effects of Trihoney on hypercholesterolaemia-induced male reproductive hormonal changes in male rabbits and compare its effects with atorvastatin. Materials and methods: Forty-eight male New Zealand white rabbits were assigned into 6 groups as follows; Control: commercial pellet; CH: commercial pellet with 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet with 0.3 g/kg/day Trihoney; DH2: 1% cholesterol diet with 0.6 g/kg/day Trihoney and DAt: 1% cholesterol diet with 2mg/kg/day atorvastatin. After 12 weeks, the rabbits were sacrificed and blood samples were collected for analysis of hormones and pro-inflammatory cytokines and calculation of HOMA-IR. The testes were homogenized for intra-testicular testosterone measurement. Results:Serum testosterone reduced significantly in HCD (p
Spermatogonial stem cells (SSCs) are classifiedas a unique adult stem cells that have capability to propagate, differentiate, and transmit genetic information to the next generation. Studies on human SSCs may help resolve male infertility problems, especially in azoospermia patients. Therefore, this study aims to propagate SSCs in-vitro with a presence of growth factor and detect SSC-specific protein cell surface markers.