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  1. Fulltext Effect of Carcinomas on Autosomal Trait Screening: A Review Article
    Alhatim H, Abdullah MNH, Abu Bakar S, Amer SA
    Curr Issues Mol Biol, 2023 Sep 04;45(9):7275-7285.
    PMID: 37754244 DOI: 10.3390/cimb45090460
    This review highlights the effect of carcinomas on the results of the examination of autosomal genetic traits for identification and paternity tests when carcinoid tissue is the only source and no other samples are available. In DNA typing or genetic fingerprinting, variable elements are isolated and identified within the base pair sequences that form the DNA. The person's probable identity can be determined by analysing nucleotide sequences in particular regions of DNA unique to everyone. Genetics plays an increasingly important role in the risk stratification and management of carcinoma patients. The available information from previous studies has indicated that in some incidents, including mass disasters and crimes such as terrorist incidents, biological evidence may not be available at the scene of the accident, except for some unknown human remains found in the form of undefined human tissues. If these tissues have cancerous tumours, it may affect the examination of the genetic traits derived from these samples, thereby resulting in a failure to identify the person. Pathology units, more often, verify the identity of the patients who were diagnosed with cancer in reference to their deceased tumorous relatives. Genetic fingerprinting (GF) is also used in paternity testing when the alleged parent disappeared or died and earlier was diagnosed and treated for cancer.
  2. Fulltext STR profiling in a cohort of Saudi patients with acute leukemia
    Alhatim HA, Abdullah MN, Jamaludin SA, Nurdin AB, Amer SA
    J Taibah Univ Med Sci, 2025 Feb;20(1):62-72.
    PMID: 40026413 DOI: 10.1016/j.jtumed.2025.01.003
    OBJECTIVE: This study was aimed at characterizing whether the autosomal short tandem repeats (STRs) used in forensic identification might differ between leukemic blood samples and saliva samples.

    METHODS: Blood and saliva samples were collected from 27 patients diagnosed with acute leukemia in Riyadh City, KSA. DNA was extracted, and 15 STR loci were amplified.

    RESULTS: Approximately 59.3% of patients with leukemia exhibited mutations at the STR loci. Loss of heterozygosity (LOH) occurred in 40.7% of the patients at D19S433, D16S539, vWA, D13S317, TH01, FGA, and D2S1338. Microsatellite instability (MSI) was detected in 22.2% of patients at TPOX, vWA, D19S433, D16S539, and D18S51. D19S433 and D16S539 were the most affected loci, exhibiting an alteration percentage of 18.52%, followed by vWA (11.11%); in contrast, D2S1338, D18S51, and TPOX were the least affected loci, showing a mutation percentage of 3.7%. D13S317, TH01, and FGA showed moderate genetic mutation (7.41%). CSF1PO, D21S11, D3S1358, D5S818, D7S820, D8S1179, and amelogenin did not show genetic changes in all samples. The overall genetic variability between saliva and blood samples significantly differed (P 

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