The plug-in hybrid electric vehicle (PHEV) has received extensive attention due to the alternatives it provides for reducing the dependency on petroleum. Nowadays, many simulation software such as ADVISOR and AUTONOMIE, were built to help in PHEV research. In this paper, the effect of three main PHEV powertrains such as engine power, traction motor power and battery capacity on fuel consumption, electric consumption and carbon oxide emission is studied using AUTONOMIE. The simulation is designed and run using AUTONOMIE with example of commands used in MATLAB. The overall simulation results show that the fuel consumption decreases as the motor power increases with the same battery capacity and engine power. In additions, the simulation results also show that the electric and fuel consumptions are contrary to each other. Meanwhile, the carbon oxide emission and fuel consumption increase following each other’s.