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  1. Muniandy KV, Chung ELT, Jaapar MS, Hamdan MHM, Salleh A, Jesse FFA
    Toxicon, 2020 Jan 30;174:26-31.
    PMID: 31989927 DOI: 10.1016/j.toxicon.2019.12.158
    Brachiaria decumbens (signal grass) is a highly productive tropical grass that is widespread in some tropical countries due to its adaptation to a wide range of environments and soil types. However, a limiting factor for the use of this grass is its toxicity from steroidal saponins. Sporadic outbreaks of hepatogenous photosensitization in ruminants grazing on this grass have been reported. Sheep are more susceptible than other animal species and the young are more susceptible than adults. This review article will critically shed light on the B. decumbens profile, its toxic compounds, mechanisms, clinical responses, blood profile alterations, pathological changes, and acute phase responses related to signal grass intoxication. Further research is needed to integrate new findings on B. decumbens intoxication with previous preventive and therapeutic trials to minimize or remove its deleterious toxic effect.
  2. Muniandy KV, Chung ELT, Jaapar MS, Hamdan MHM, Reduan MFH, Salleh A, et al.
    Trop Anim Health Prod, 2021 Jun 25;53(3):372.
    PMID: 34173068 DOI: 10.1007/s11250-021-02820-1
    The present study aims to determine the hematology, serum biochemistry, and acute phase proteins (APPs) responses of both serum and cerebrospinal fluid (CSF) in sheep fed with low and high levels of Brachiaria decumbens (B. decumbens) diets at different time phases. A total of 30 6-month-old male Dorper cross sheep were randomly divided into three treatment groups consisted of 10 sheep each. Treatment 1 (control) sheep were fed with Pennisetum purpureum and concentrates as the basal diet, whereas Treatments 2 and 3 sheep were fed with low (10%) and high (60%) level of B. decumbens, respectively. The hematology results revealed that there were significant differences (p 
  3. Jaapar MS, Chung ELT, Nayan N, Muniandy KV, Hamdan MHM, Jusoh S, et al.
    Trop Life Sci Res, 2023 Mar;34(1):67-83.
    PMID: 37065792 DOI: 10.21315/tlsr2023.34.1.5
    Limited data are available regarding the effects of Brachiaria decumbens on sheep's growth performance at different times. Therefore, this current study focused on sheep's nutrient apparent digestibility, feed efficiency, body index, and growth hormone when they are fed with low and high levels of B. decumbens diets. A total of 30 six-month-old male Dorper cross sheep were divided randomly into three treatment groups with 10 sheep per treatment. Treatment 1 (control) sheep were fed with Pennisetum purpureum and pellets as the basal diet, whereas Treatment 2 and 3 sheep were fed with feed mixed with low (10%) and high (60%) levels of B. decumbens, respectively. The study was conducted in two phases consisting of short-term feeding (seven days) and long-term feeding (90 days). Throughout the experiment, daily fecal voided were collected in the morning for seven days continuous before the end of each feeding phases for the determination of nutrient apparent digestibility. The amount of feed offered and refusals plus body weight gain were recorded daily to determine the feed efficiency (FE). Besides, the body measurements of each sheep from every treatment were measured weekly and blood samples were collected for the analysis of growth hormone (GH) concentration. There were significant differences (p < 0.05) in the nutrient apparent digestibility, growth performance, body measurement, and GH concentration among treatment sheep throughout the study period. Treatment 3 sheep fed with 60% of B. decumbens diet revealed the lowest dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility during the long-term feeding. Likewise, Treatment 3 (T3) sheep had the lowest total bodyweight gain, average daily gain, total feed intake, and daily feed intake among treatment sheep. The heart girth index (HGI) of T3 sheep was also significantly lower during the short-term feeding. Moreover, the GH concentration of T3 sheep was significantly lower as compared to the control that decreases steadily throughout the study period. In conclusion, high levels of B. decumbens showed the most significant results out of all three treatments indicating the presence of saponins, which produce negative effects on the sheep's overall performance.
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