Four endophytic fungi have been tested for antioxidant properties using different assays; DPPH radicalscavenging activity, ferric reducing antioxidant power (FRAP) and ferrous ion chelating activity (FCA). The test of polyphenolic content also has been done for both total phenolic content (TPC) and total flavonoid content (TFC). There was no result on the half maximal concentration (IC50) for both DPPH and FCA assays for all fungi. However, through FRAP assays the results were ranged from 0.336±0.01 to 0.477±0.11 mmol Fe2+/g extract where N. sphaerica had the highest result. This fungus also showed the highest results on TPC and TFC, which were 0.030±0.000 (mg GAE/g) and 0.038±0.001 (mg QE/g) respectively.
The glutathione S-Transferase (GST) enzyme plays an important role in cellular detoxification. This multifunctional enzyme
is involved in Phase II detoxification pathways that protect cellular macromolecules from being attacked by harmful
compound. The study is an attempt to isolate glutathione transferase-expressing bacteria from the rhizospheric soil of
selected herbal plants. Screening showed nine positive isolates out of twelve bacterial samples from a large microbial
population in our soil collection. Crude extract from strain E1 which was isolated from Piper sarmentosum (Kadok)
showed the highest specific activity against 1-chloro-2, 4-dinitrobenzene substrates (5.78 × 10-06 µmol/min/mg). Based
on the carbon utilization of E1 assessed using Biolog system, the strain was identified as Comamonas testosterone E1.
Glutathione S-transferase purification using GST trap yielded two distinct subunits with molecular weights of 23 and 24
kDa as visualized on 1D SDS-polyacrylamide gel electrophoresis. The purified GST showed reactivity towards 1-chloro-2,
4-dinitrobenzene, 1, 2-dichloro-4-nitrobenzene and ethacrynic acid with specific activity of 0.264 ± 0.038 nmol/min/
mg and 0.056 ± 0.002 nmol/min/mg and 10.500 ± 3.130 nmol/min/mg, respectively. However, no activity was detected
against p-Nitrobenzyl chloride, Sulfobromophthalein, trans-4-phenyl-3-butene-2-one, hexa-2, 4- dienal, trans-hepta-2,
4-dienal and trans-oct-2-enal in the study.