Introduction: The application of nanofibers in biomedicine has gained increasing interest due to their characteristic large surface area, high porosity and chemical tunability. Electrospinning is one of the most versatile techniques for the production of uniform nanofibers. It has been used for the fabrication of extracellular matrix (ECM)-mimicking fibrous scaffolds for several decades. Electrospun fibrous scaffolds provide nanoscale to microscale fibrous structures with interconnecting pores, resembling natural ECM in tissues, and showing a high potential to facilitate the formation of artificial functional tissues. Furthermore, electrospinning demonstrated potential as a vehicle for the encapsulation of bioactive compounds such as phenolic acids, which was suggested to have significant potential to treat wounds caused by trauma, diabetes, ischemic syndromes and other pathological diseases. This study investigates the application of electrospun nanofibers as bioactive carriers of phenolic acids, where gallic, caffeic and p-coumaric acids were incorporated to zein nanofibers at different concentrations (5%, 10% and 20%). Methods: The morphology of the produced fibers were examined with scanning electron microscopy (SEM) and exhibited diameters ranging from 396 to 655 nm. Meanwhile, the interaction between the phenolic compounds and zein was examined with attenuated total reflection-Fourier transform infrared (ATR-FTIR). Results: The antioxidant activity of the fibers was determined using 1,10-diphenyl-2-picrylhydrazyl (DPPH) assay and showed that gallic and caffeic acid had retained their active properties after the incorporation to zein electrospun fibers. Conclusion: Overall, the study provides an outline on the potential of electrospinning technique to produce nanofibers that may serve as substrates in skin tissue engineering and carriers for bioactive compounds such as phenolic acids.