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  1. Fulltext The Antioxidant Activity and Induction of Apoptotic Cell Death by Musa paradisiaca and Trigona sp. Honey Jelly in ORL115 and ORL188 Cells
    Anuar MNN, Ibrahim M, Zakaria NH, Ichwan SJA, Md Isa ML, Mat Alewi NA, et al.
    Malays J Med Sci, 2023 Feb;30(1):82-91.
    PMID: 36875196 DOI: 10.21315/mjms2023.30.1.7
    BACKGROUND: Head and neck cancer patients usually need nutritional support due to difficulties in swallowing and chewing. Therefore, this study aimed to formulate Musa paradisiaca and Trigona sp. honey jelly (MTJ) as a convenient functional food.

    METHODS: The antioxidant properties were analysed using 2,2'-diphenyl-1 picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP) and 2,2'-azinodi 3-ethylbenthiazolinesulfonate (ABTS) assays. Cytotoxicity was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and the induction of apoptosis was observed via caspase-3/7 activity assay. The identification of phenolic compounds was done via ultra-high-performance-liquid chromatography coupled to mass spectrometer (UHPLC-MS/MS).

    RESULTS: The antioxidant analysis exhibited: the half inhibitory concentration (IC50) of DPPH inhibition, 54.10 (SD = 4.51) μg/mL; the FRAP value, 30.07 (SD = 0.93) mM TEQ/100 g; and the ABTS value, 131.79 (SD = 8.73) mg TEQ/100 g. Cinnamic acid was the most abundant phenolic compound, followed by maleic acid and salicylic acid. The IC50 for ORL115 and ORL188 were 35.51 mg/mL and 43.54 mg/mL, respectively. The cells became rounded and dissymmetrical which reduced in number and size. The apoptotic cell death in ORL115 and ORL188 was deduced as caspase-3/7 activities that significantly increased (P < 0.05).

    CONCLUSION: The study evidenced that the antioxidant activity of MTJ could influence the induction of apoptosis in ORL115 and ORL188 in future investigations and verifications.

  2. Refinement of Synthetization Parameters for High Laccase-Like Activity of Imidazole-Copper (II) Nitrate Trihydrate Nanozyme Towards an Efficient Biomimetic Nanozyme
    Mat Alewi NA, Rahman RA, Md Illias R, Jaafar NR, Abd Rahman NH, Chia BJ, et al.
    Appl Biochem Biotechnol, 2025 Mar 26.
    PMID: 40138139 DOI: 10.1007/s12010-025-05229-w
    Laccase's industrial application is hindered by its sensitivity and low stability to extreme conditions. To overcome these limitations, the development of biomimetic nanozymes is gaining momentum. Nevertheless, developing multifunctional nanozymes with high laccase-like activity poses several challenges. This study focused on optimizing the synthesis of imidazole-copper (II) nitrate trihydrate (I-Cu) nanozymes and characterizing its physicochemical properties. Key synthesis parameters (precursor amount, incubation time, and oven temperature) were optimized. I-Cu nanozymes were synthesized in a Teflon-lined autoclave via water-induced precipitation of Cu2+ and imidazole, mimicking the N-Cu coordination found in laccase's active sites. Initial screenings revealed the superior catalytic activity of I-Cu nanozymes synthesized using methanol compared to ethanol, and a smaller nano-scale size than laccase. FTIR analysis confirmed the presence of similar chemical components as laccase (C44H69N11O20), verifying I-Cu nanozyme's capability to degrade phenolic compounds, and imidazole did not decompose throughout the synthesis process. The optimized I-Cu nanozyme demonstrated higher catalytic activity (6.569 UA), oxidation efficiency (Vmax of 0.00893 mM/min and Km of 2.4020 mM), and greater stability under varying pH, temperature, and storage conditions, compared to laccase. Conclusively, the optimized I-Cu nanozyme, with a 6.00-fold increase in catalytic activity compared to previous studies, as well as 1.69-fold higher Km, and 2.08-fold higher Vmax compared to laccase, shows promise as a wastewater treatment alternative. Its enhanced performance, achieved with fewer precursors through synthesis optimization, highlights the potential of lesser-known biomimetic nanozymes and underscores the importance of refining the synthesis parameters.
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