Although electrospun poly(methyl methacrylate) (PMMA) may mimic structural features of extracellular matrix, its highly
hydrophobic nature causes reduced cell attachment. This study analysed the physicochemical and structural changes
of the surface modified PMMA nanofiber. The electrospun PMMA nanofibers (PM) were surface-treated as follows: PM
alone, collagen coated-PM (PM-C), UV-irradiated PM (PM-UV), collagen coated UV-irradiated PM (PM-C-UV) and collagen
coated-PM crosslinked with genipin (PM-C-GEN). They were subjected to scanning electron microscopy, Fourier transform
infrared (FTIR), cell attachment analysis, X-ray photoelectron spectroscopy (XPS), atomic force microscopy and X-ray
powder diffraction (XRD). The surface roughness was lower in PM-C-UV group compared to others. Based on FTIR
results, all expected functional group were present in all groups. XPS result showed that there are changes in the mass
concentration of UV-treated surfaces and in the collagen coated surfaces. All PM groups showed amorphous nature through
XRD. UV irradiation and collagen coating were shown to increase PM’s functional groups and modify its surface, which
contributed to the increased attachment of cells onto the inert PM scaffold. As conclusion, collagen coated UV irradiated
PMMA provided a better surface for cell to attach hence are suitable to be used further as scaffold for in vitro model.