Amaranthus spinosus Linn. complete plant material was extracted successively by petroleum ether, chloroform, methanol, and water. All the extracts were subjected to in vitro non-enzymatic haemo-glycosylation method, i.e. an oxidation reaction. The degree of haemoglycosylation in the presence of different extracts of Amaranthus spinosus were measured colorimetrically at 520 nm. The preventive effect of haemoglobin glycosylation at the two concentration; 0.5 and 1 mg/mL was estimated as follows: pet. ether; 13.1%, 16.4%, chloroform; 5.7%, 12%, methanol: 36.91%, 56.07% and aqueous: 22.2%, 31.01 %, respectively. The α-tocopheral (Vitamin E) was used as standard.
Nisamalaki Churna is an Ayurveda formulation used for diabetes, which consists of amalaki and haridra. in vitro antidiabetic screening of Nisamalaki Churna by α-amylase inhibition was carried out by starch iodine method, dinitrosalicylic acid method (DNSA) and effect on normal rats blood sugar level was studied. Nisamalaki Churna shows potent α-amylase inhibition IC50 89.44 µg/mL by starch iodine method and IC50100.0 µg/mL by DNS method. Nisamalaki Churna showed potent decrease in blood glucose level in normal rats.