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  1. Photodynamic antibacterial activity of green-synthesized gold nanoparticles from mangosteen peel extract using blue laser irradiation
    Arifianto D, Astuti SD, Lusiani BM, Zaidan AH, Winarno W, Yaqubi AK, et al.
    Lasers Med Sci, 2025 Jun 30;40(1):305.
    PMID: 40583083 DOI: 10.1007/s10103-025-04549-x
    INTRODUCTION: diseases caused by antibiotic-resistant bacteria pose a growing global health challenge. Photodynamic Therapy (PDT), which uses light-activated photosensitizers to kill bacteria, offers a promising alternative. This study evaluates the antibacterial efficacy of gold nanoparticles synthesized from mangosteen peel extract (AuNPs-GML) combined with blue diode laser irradiation against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), two common pathogens.

    MATERIALS AND METHODS: Four treatments were applied: control (no treatment), AuNPs-GML alone, blue laser irradiation alone (405 nm), and a combination of AuNPs-GML with laser irradiation. Samples were incubated at 37 °C for 24 h before bacterial viability was assessed. Statistical analysis was performed using Two-Way ANOVA and Tukey's post hoc test (p coli and 72.84% for S. aureus at an energy density of 3.34 J/cm2.

    CONCLUSION: This study demonstrates the potential of AuNPs-GML activated by blue laser PDT as an effective antibacterial treatment against resistant bacteria.

    Matched MeSH terms: Escherichia coli/radiation effects
  2. Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein
    Huwaidi A, Pathak N, Syahir A, Ikeno S
    Biochem Biophys Res Commun, 2018 09 05;503(2):910-914.
    PMID: 29928878 DOI: 10.1016/j.bbrc.2018.06.095
    Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide showed a higher number of colony-forming units per dilution under UVA and UVC treatment. These results suggested that expression of the short peptide could be useful for the development of genetically modified organisms and in applications that require resilience of organisms to UV radiation.
    Matched MeSH terms: Escherichia coli/radiation effects*
  3. Proton-Functionalized Graphitic Carbon Nitride for Efficient Metal-Free Destruction of Escherichia coli under Low-Power Light Irradiation
    Ng BJ, Musyaffa MK, Er CC, Packiam KAR, Lee WPC, Tan LL, et al.
    Chemistry, 2021 Feb 10;27(9):3085-3090.
    PMID: 33263935 DOI: 10.1002/chem.202004238
    Universal access to clean water has been a global ambition over the years. Photocatalytic water disinfection through advanced oxidation processes has been regarded as one of the promising methods for breaking down microbials. The forefront of this research focuses on the application of metal-free photocatalysts for disinfection to prevent secondary pollution. Graphitic carbon nitride (g-C3 N4 ) has achieved instant attention as a metal-free and visible-light-responsive photocatalyst for various energy and environmental applications. However, the photocatalytic efficiency of g-C3 N4 is still affected by its rapid charge recombination and sluggish electron-transfer kinetics. In this contribution, two-dimensionally protonated g-C3 N4 was employed as metal-free photocatalyst for water treatment and demonstrated 100 % of Escherichia coli within 4 h under irradiation with a 23 W light bulb. The introduction of protonation can modulate the surface charge of g-C3 N4 ; this enhances its conductivity and provides a "highway" for the delocalization of electrons. This work highlights the potential of conjugated polymers in antibacterial application.
    Matched MeSH terms: Escherichia coli/radiation effects*
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