Displaying publications 21 - 25 of 25 in total

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  1. Yadav, M., Harnam, S.
    MyJurnal
    The total and allergen-specific IgE response of patients with rhinitis, rhinoconjunctivities, rhinitis with asthma and rhinitis with dermatitis was analyzed to a panel of twelve high risk airborne and food allergens. It has been found that this panel will detect 96 percent of the allergen-causing diseases in children. It was found that 26 (76%) of the 34 young patients had a family history for atopy suggesting a high frequency of inheritance of allergic disorders. Elevated total IgE was found in most patients with allergic disease. Positive IgE antibody response to two species of Dermatophagoides mite-allergens was found in 7/12 (58%) patients with rhinitis, 11/11 (100%) patients with rhinoconjunctivitis, 7/9 (78%) cases ofrhinitis with asthma and 3/3 (100%) cases of rhinitis with dermatitis. Some of the patients also responded to food allergens. Food allergy was noted in 4/12 (33%) cases of rhinitis, 11/11 (100%) cases of rhinoconjunctivitis, 4/9 (44%) cases of rhinitis with asthma and 1/3 (33%) cases of rhinitis with dermatitis. Patients with rhinoconjunctivitis who tested positive at high titres for mites invariably had enhanced response for cockroaches, shrimps and crabs suggesting invertebrate antigen cross-reactivity. A few patients however, did not show this type of cross-reactivity although they had high titres of anti-mite IgE antibodies indicating that they responded to non cross-reacting allergen epitopes. Response to multiple allergens appears to be a common feature of most patients with respiratory or skin allergic diseases. The prevalence of multiple target-organ allergy to wide variety offood and environmental allergens complicates the long term management ofpatients with such allergic disorders.
    Matched MeSH terms: Cockroaches
  2. Akbar N, Siddiqui R, Iqbal M, Sagathevan K, Khan NA
    Lett Appl Microbiol, 2018 May;66(5):416-426.
    PMID: 29457249 DOI: 10.1111/lam.12867
    Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii. The results revealed that the gut bacteria of cockroaches produce active molecule(s) with potent antibacterial properties, as well as exhibit antiamoebic effects. However, heat-inactivation at 95°C for 10 min had no effect on conditioned media-mediated antibacterial and antiamoebic properties. These results suggest that bacteria from novel sources i.e. from the cockroach's gut produce molecules with bactericidal as well as amoebicidal properties that can ultimately lead to the development of therapeutic drugs.

    SIGNIFICANCE AND IMPACT OF THE STUDY: The bacteria isolated from unusual dwellings such as the cockroaches' gut are a useful source of antibacterial and antiamoebal molecules. These are remarkable findings that will open several avenues in our search for novel antimicrobials from unique sources. Furthermore studies will lead to the identification of molecules to develop future antibacterials from insects.

    Matched MeSH terms: Cockroaches/microbiology*
  3. Huang CH, Liew LM, Mah KW, Kuo IC, Lee BW, Chua KY
    Clin Exp Allergy, 2006 Mar;36(3):369-76.
    PMID: 16499649
    Sensitization to mite and cockroach allergens is common, and diagnosis and therapy of allergy can be further complicated by the presence of allergen isoforms and panallergens. Purified recombinant and native allergens are useful for studies to resolve such problems.
    Matched MeSH terms: Cockroaches/immunology*
  4. Soegiarto G, Abdullah MS, Damayanti LA, Suseno A, Effendi C
    Asia Pac Allergy, 2019 Apr;9(2):e17.
    PMID: 31089459 DOI: 10.5415/apallergy.2019.9.e17
    Background: The prevalence of allergy among Surabaya school children is currently unknown.

    Objective: To identify the prevalence of the common allergic sensitization and allergic diseases among school children and undergraduate students in suburban of Surabaya by epidemiologic data collection.

    Methods: A multistage simple random sampling was done to select 5 primary schools, 8 secondary schools (4 of junior high schools and senior high schools, respectively), and 1 university from 5 districts in Surabaya city. Out of 550 invited respondents, 499 (128 primary school, 221 secondary school, and 150 undergraduate) respondents gave their consent. A complete personal history, allergic symptoms, environmental exposure of common allergens was obtained from interview and the physical examinations were performed. Skin prick test (SPT) was done using 45 different allergen extracts. Total serum IgE and specific IgE radioallergosorbent test levels were measured for respondents with allergic manifestations.

    Results: There was an increasing SPT positivity among study respondents, from primary school, secondary school, to undergraduate students (21.90%, 28.95%, to 45.30% respectively). Cockroach (42.85%) and fungi/mold spore (42.85%) were the most common allergens in primary school children. House dust mites was the most common allergen in secondary school (63.16%) and undergraduate students (58.82%). Urticaria and rhinitis were the commonest allergic diseases manifestation. History of atopy was positive in 60.79% of the allergic respondents.

    Conclusion: The prevalence of allergic sensitization among school children and undergraduate students in Surabaya suburb areas were increased compared to previous estimates in 1998. While house dust mites are known as important allergens, surprisingly cockroach was the common allergen among the younger school children.

    Matched MeSH terms: Cockroaches
  5. Ali SM, Siddiqui R, Sagathevan KA, Khan NA
    Folia Microbiol (Praha), 2021 Apr;66(2):285-291.
    PMID: 33704690 DOI: 10.1007/s12223-021-00860-6
    The evolution of multiple-drug resistant bacteria is contributing to the global antimicrobial crisis, hence driving us to search for novel antimicrobial(s). Among animals, invertebrates represent up to 80% of all known species suggesting their wide distribution. Despite their ubiquitous and plentiful nature, they have been largely unexplored as potential source of antibacterials. In this study, we selected a broad range of invertebrates from terrestrial and marine environments and tested their lysates for antibacterial activity against methicillin-resistant Staphylococcus aereus (MRSA) and neuropathogenic Escherichia coli K1. Cockroaches, centipedes, tarantulas, prawns, lobster, and mud crabs showed antibacterial activity with selected lysates exhibiting more than 90% bactericidal effects. The red-headed centipede's hemolymph showed 90% and 50% bacteriostatic activity against MRSA and E. coli K1, respectively. Tarantula's body extracts exhibited antibacterial activity against MRSA and E. coli K1. Gut extracts of tiger prawn exhibited more than 90% bacteriostatic activity against both bacteria. The selected lobster and mud crab extract exhibited up to 90% growth inhibitory activity against MRSA. Overall, these results showed that selected invertebrates are an untapped source of broad-spectrum antibacterial activity and suggest the presence of biologically active molecules.
    Matched MeSH terms: Cockroaches
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