The Malaysian Adults Nutrition Survey (MANS) was carried out between October 2002 and July 2003, involving 6,775 men and 3,441 women aged 18 - 59 years. Anthropometric assessment showed that the overall mean body weight and BMI were 62.65 kg (CI: 62.20, 63.09) and 24.37 kg/m2 (CI: 24.21, 24.53) respectively. Based on the WHO (1998) classification of BMI, 12.15% (CI: 11.26, 13.10) were obese (BMI > 30 kg/m2), and 26.71% (CI: 25.50, 27.96) overweight (BMI > 25 - 29.9 kg/m2). Significantly, more women were obese [14.66% (CI: 13.37, 16.04)] while significantly more men were overweight [28.55% (CI: 26.77, 30.40)]. Ethnicitywise, prevalence of obesity was highest among the Malays [15.28% (CI: 13.91, 16.77)] while overweight was highest for the Indians [31.01% (CI: 26.64, 35.76)]. Both obesity and overweight were highest among those aged 40 - 49 years. Obesity was highest for those whose household income was between RM1,500 - 3,500 while overweight was more prevalent for those whose household income exceeded RM3,500. The prevalence of overweight was highest for those with primary education [31.90% (CI: 29.21, 34.72)]. There was no significant urban rural differential in both obesity and overweight. The study found 9.02% (CI:8.82, 10.61) with chronic energy deficiency (CED) (BMI < 18.5 kg/m2). The prevalence of CED was relatively higher in the indigenous population (Orang Asli) [14.53% (CI: 5.14, 34.77)], subjects aged 18-19 years [26.24% (CI: 21.12, 32.09)], and with monthly household income of < RM1,500 [10.85% (CI: 9.63, 12.20)]. The prevalence of CED was not significantly different among the geographical zones and educational levels, and between urban/rural areas and sexes. The results call for priority action to address the serious problem of overweight and obesity among Malaysian adults as it poses a grave burden to the country's resources and development.
Study name: Malaysian Adult Nutrition Survey (MANS-2003)
Body fatness distribution is a useful epidemiological and clinical marker of health risk among European and other populations. Prevalence of obesity among adults is rising in many parts of Malaysia, and it is important to understand the extent to which fat patterning is robust across different age groups and between the sexes. This analysis examines fatness and fat distribution in rural Sarawakian adults aged 20 to 80 years to determine the extent to which fat patterning varies by sex and age. Principal component analysis of five skinfolds (biceps, triceps, subscapular, suprailiac and medial calf) shows upper body-lower body fat distribution as contributing most to within-group variation in the sum of five skinfolds for both males and females. When divided into younger and older age groups, clear differences were identified. For the males, while absolute fatness does not change with age, there is a change in fat patterning, with a trunk-extremity component disappearing, and a trunk-lower body pattern persisting. Females experience a decline in subcutaneous fatness across age groups, and while the most important component of fat distribution, trunk to lower body subcutaneous fatness, the same for younger and older women, the second component is different, with upper limb-lower body distribution in the younger age group being replaced with upper to lower trunk distribution. The similarities in fat patterning among males and females suggest that the use of fat patterning indices in this Malaysian population need not be sex-specific, but age group- and sex-dependent differences in the second principal component indicate that they should be used with caution, since the relationships between fat patterning and mortality are still poorly understood among Malaysian populations.
There has been a change in the lifestyles of populations, including reduced physical activity and consumption of foods high in calories. Overweight and obesity are now replacing the more traditional public health concerns such as under-nutrition and infectious diseases as some of the most significant contributors to ill health. Determination of the body mass index (BMI) profile and nutritional status of adults of two rural coastal villages in Northern Malaysia was part of a community diagnosis in a community survey. Height and weight were measured and BMI calculated. Blood pressure was measured using a manual sphygmomanometer according to WHO guidelines. A standardised questionnaire was used to interview the villagers concerning their health. Out of the total population, 504 were above 20 years of age. Data was available for 441 persons for analysis. There were 210 (47.6%) males and 231 (52.4%) females. The prevalence of underweight was 9.8% (n=43), overweight 25.9% (n=114) and obesity 17% (n=75). The problem of over-nutrition was significantly higher among females, especially housewives. (p< 0.05). Those in ages 41-70 years were the majority with problems of over-nutrition (p=<0.05). More than half (52.9%; n=39) of those who were obese had hypertension (p=<0.05). Results show that a higher number of women especially housewives were obese and more than half of those obese subjects had hypertension. A more thorough nutritional profile using waist, hip and body fat measurement as well as an assessment of the dietary intake and activity regime of these villagers is needed. Interventions need to be carried out before more serious complications of obesity become rooted in this community.
Introduction: High sensitivity C-reactive protein (hs-CRP) is an emerging risk marker for cardiovascular diseases (CVD). In Malaysia, CVD has become a major health problem and the risk factors of CVD have also increased among the middle- aged. Thus, this study aimed to determine factors that influence the level of hs- CRPamongMalaysianadultsaged30-55years-old. Methods:One-hundredand twenty-two (n=122) adults working at an institution were selected systematically in this cross-sectional study. Body weight, height, hip and waist circumference, systolic and diastolic blood pressure, hs-CRP level, total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein were measured. Body mass index (BMI), waist-to-hip ratio, and total cholesterol: high-density lipoprotein ratio were calculated. Results: The sample consisted of 40.2% male and 59.8% female subjects with a mean age± SD of 41.93 ± 8.26 years. Pearson's correlation coefficient showed significant positive correlations between log hs-CRP level (mean± SD= 0.22 ± 0.50 mg/L; 95% CI= 0.13 - 0.31) and age, waist circumference, hip circumference, BMI, systolic blood pressure, total cholesterol, low-density lipoprotein, triglycerides, and total cholesterol: high-density lipoprotein ratio. However, multivariate analysis showed only BMI (R = 0.489, R2 = 0.239, adjusted R2 = 0.232, F = 37.626, p< 0.001) was a predictor of hs-CRP, the risk marker of CVD. Hs-CRP level was greater in overweight (25 - 29.9 kg/m2), and obese(> 30 kg/ m2) subjects (ANOVA p< 0.05) compared to normal weight subjects. Conclusion: BMI is a modifiable risk factor with the change being important for reducing CVD events among adults.
The recommendation to lose weight has been the guiding principle in the treatment of type 2 diabetic patients. However, to facilitate this process, it is vital to understand factors associated with personal feelings about body weight and related behaviour.
A cross-sectional study was conducted to determine bone health status and nutrient intakes among post-menopausal women residing in low cost houses in Cheras, Kuala Lumpur.
An analytical cross-sectional study was conducted in a public university in Kuala Lumpur among a random sample of 2665 undergraduates. The objective was to study the prevalence of breakfast skipping and its associated factors. Data collection was conducted via a self-administered pre-tested questionnaire. There were 43.5% male respondents, with Malays being the majority (58.3%). The prevalence of breakfast skipping was 29.2 (95% CI: 27.3 - 30.3)%. The factors significantly associated with breakfast skipping (p<0.05) were age, race, accommodation, faculty and skipping dinner. As the respondents' age increased, their risk of breakfast skipping was lower (OR: 0.95; 0.89 - 0.99). Malays (OR: 1.94; 1.48 - 2.54), Indians (OR: 1.70; 1.08 - 2.66), and students from the Sabah and Sarawak indigenous communities (OR: 2.13; 1.37 - 3.33) were more likely to skip breakfast compared to their Chinese counterparts. Respondents who stayed in their own houses were also less likely to skip breakfast compared to those staying in hostel with meals catered (OR: 2.32; 1.39 - 3.84), hostel with cafeteria (OR: 2.92; 1.74 - 4.91) or in rented houses (OR: 2.08; 1.25 - 3.46). Respondents majoring in Arts and Economics had 1.40 (1.07 - 1.82) times risk of breakfast skipping compared to those majoring in Life Sciences. Those who skipped dinner too had twice the odds (1.47 - 2.77) of breakfast skipping. In conclusion the prevalence of breakfast skipping among the undergraduates of this university was moderately high. Health awareness campaigns or introduction of healthy eating guidelines should be initiated for the undergraduates as well as food caterers in campus. The policy and pricing of catered food in campus should also be reviewed.
It is universally known that breastfeeding provides a broad spectrum of nutritional and non-nutritional advantages to the developing infant. Nonnutritive protecting potentials of breast milk towards different infections and non-infectious diseases are still attracting the attention of researchers in different specialties. The neonate, who is suddenly exposed to a wide variety of organisms, is in dire need of protection, enhancement and education of his immature immune system to encounter these organisms. The lactating mammary gland is an integral part of the common mucosal immune system which stands as a sentinel in combating pathogens that enter the body via the mucosal route. The common mucosal immune system also competently controls tolerance mechanisms to innocent proteins and is involved in surveillance of carcinogensis. The diverse roles of general mucosal immunity are nearly well established but the specialised functions of breast tissue and breast milk in boosting the immune responses need more emphasis and highlighting. The detailed understanding and evaluation of breast milk as an immunological tool is reviewed within the domain of the diverse activities of the common mucosal immune system.
A survey was carried out in the district of Kemaman, Terengganu, Malaysia to study infant feeding practices in rural and semi-urban communities. A total of 593 mothers were interviewed and their socio-demographic information recorded. Data on infant feeding practices were collected from mothers who had children up to 15 months of age. Among breast-feeding mothers (n =157), 42.0 % fed their babies for less than 3 months and 58.0% bottle fed for more than 6 months. Sixteen children were found to be fed on sweetened condensed milk with 62.5% of them for the first 3 months. Among those who breast fed their babies, 40.1% were found to have bottle fed at one time or another. The majority of breast feeding mothers belonged to the groups having incomplete primary schooling or completed primary education only and household income below RM600 per month. A substantial number of breast fed babies were given weaning foods in the form of porridge mixture (rice + egg, rice + vegetables, rice + meat, rice+ fish and cereals) between the age of 0 - 3 months. The findings of this study concluded that although breast-feeding is widely practiced, however, their duration has dwindled, and early introduction of solid foods is widespread.
A survey of infant-feeding and weaning practices of 566 mothers, systematically sampled from 15 rural villages, randomly selected in the district of Tumpat, Kelantan was carried out. Almost all mothers (97.3%) breastfed their children, reinforcing the previously reported high incidence of breast-feeding among rural Malaysian mothers. One hundred and seventeen (21.3%) of the 551 children breastfed were also given mixed feeding with infant fomulae as well. Weaning started before 4 months of age in 28.3% of the children and after 6 months of age in 12.8% of the children. The 3 most common type of food used in weaning were Nestum (45.0%), rice porridge or paste (42.6%) and wheat porridge or cakes (11.5%). Forty eight children (12.1%) discontinued breastfeeding once weaning was initiated. The main reasons for initiating weaning was mothers' perception that there was insufficient milk and that the child was always hungry (55.7%). Most mothers were also given advice by the health clinic staff on weaning, including the timing and the suitable weaning foods to give. All of the mothers were able to name at least one commercial weaning food product available in their community.
In Malaysia, the National Breastfeeding Policy recommends exclusive breastfeeding for the first six months of life and continued up to two years. Since the 1990s, several breastfeeding promotion programmes had been implemented in the country. This article reports the findings on the prevalence of breastfeeding practice from The Third National Health and Morbidity Survey (NHMS III) which was conducted in 2006. A total of 2167 mothers or carers of children below two years old were interviewed representing 804,480 of the estimated population of children aged below 2 years in Malaysia. Respondents were asked whether various types of liquid or solid food were given to the child at any time during the preceding 24-hour period. The overall prevalence of ever breastfed among children aged less than 12 months was 94.7% (CI: 93.0 - 95.9). The overall prevalence of exclusive breastfeeding below 6 months was 14.5% (CI: 11.7 - 17.9). Prevalence of timely initiation was 63.7% (CI: 61.4 - 65.9) and the continued prevalence of breastfeeding up to two years was 37.4% (CI: 32.9 - 42.2). The findings suggest that the programmes implemented in the last ten years were effective in improving the prevalence of ever breastfeeding, timely initiation of breastfeeding and continued breastfeeding up to two years. However, the challenge is to improve exclusive breastfeeding practice. Longterm community-based interventions need to be carried out in partnership with the existing health care system, focusing on discouraging the use of water and infant formula, especially in the first few months of life.
Study name: National Health and Morbidity Survey (NHMS-2006)
Introduction: The benefits of breastfeeding for both mothers and infants are
widely recognised. Breastfeeding confirms a woman’s unique ability to care for her
infant in the best way possible and promotes optimum infant and maternal health.
Methods: A qualitative research method involving five focus group discussions
(n=33) was chosen in this study to compare and contrast the breastfeeding practice
in two different locations: the communities of Pos Pulat and the regroupment
scheme settlement at Rancangan Pengumpulan Semula (RPS) Kuala Betis in
Kelantan, Malaysia which represents different lifestyle experiences of indigenous
Temiar population. Results: The benefits of breastfeeding to the infants reported
by some Temiar women (42.4%) were for the infant’s health and growth. Responses
from urban RPS Kuala Betis women include breast milk contains antibodies (3.0%),
delays in the return of regular ovulation (6.1%), thus lengthening birth intervals
and bonding between maternal-baby (6.1%). In general, respondents from Pos
Pulat seemed to have little knowledge regarding this issue, except for a woman who
mentioned that maternal milk contains vitamins. Based on the narrative analysis,
knowledge gap was observed between these two communities. Conclusion: Although
all the women interviewed had the experience of breastfeeding their infants, most
of them lacked the knowledge regarding the benefits of the breastfeeding either
to the infants or to the mothers. The findings from this study are crucial for the
preservation of breastfeeding culture among the Temiar women and can be used to
improve promotion of breastfeeding to other Orang Asli groups in Malaysia.
Thirty-two samples of chocolate products were analysed by HPLC for caffeine and theobromine contents. Defatted residues of samples were extracted with 80% aqueous acetone. After extraction into boiling water, the methylxanthines were identified and quantified with the use of μ-Bondapak column and mobile phase of methanol:water:acetic acid (20:79:1). Levels of caffein and theobromine in 32 samples of chocolate products averaged 0.62-1.14 mg/g and 0.026-0.153 mg/g respectively. Mean values for theobromine and caffeine content for chocolate coating were 0.82 and 0.07 mg/g respectively. The chocolate coating made from fat substitute had theobromine and caffeine levels ranging from 0.36-0.70 mg/g and 0.027-0.061 mg/g respectively, with mean values of 0.49 mg theobromine/g and 0.039 mg caffeine/g. In local chocolate, the mean theobromine and caffeine levels respectively were 0.72 mg/g and 0.04 mg/g in milk chocolate, and 0.85 mg/g and 0.06 mg/g in dark chocolate. Meanwhile, for imported chocolate, the mean theobromine and caffeine levels respectively were 1.05 mg/g and 0.12 mg/g in dark chocolate; 0.76 mg/g and 0.04 mg/g in milk chocolate; and 0.74 mg/g and 0.03 mg/g in white chocolate. Compared with the local chocolates, imported chocolates had higher levels of theobromine and caffeine at 1.141 mg/g and 0.1533mg/g. The average theobromine and caffeine concentrations in local chocolate were 0.082mg/g and 0.066mg/g. Theobromine concentration in chocolate samples is within the range of 0.62mg/g-1.141mg/g and the range of caffeine concentration is 0.026mg/g-0.153mg/g respectively. Bittersweet chocolates were found to have higher theobromine and caffeine concentrations than normal sweet chocolates and milk chocolates.
Bone health status was investigated in 178 free-living Chinese post-menopausal women in Kuala Lumpur. Body mass index (BMI), body composition (using whole body DXA), calcium intake and serum 25-OH vitamin D status were measured along with biochemical markers of bone turnover, that is, pro-collagen Type 1 N-terminal peptide (P1NP), osteocalcin (OC) and C-telopeptide ß cross
link of Type 1 collagen (CTX- β). Bone mineral density (BMD) was measured using DXA (Hologic, USA) at the lumbar spine, femoral neck and total hip. Results showed that osteopenia was present in 50% of the subjects at the spine and 57.9% at the femoral neck. Osteoporosis was diagnosed in 10% of the subjects at both the femoral neck and spine. A total of 29.3% of the subjects had high
levels of CTX- ß. Mean serum level of 25-OH vitamin D was 60.4+15.6 nmol/L and 50.6% of the subjects had hypovitaminosis D (defined as
An interview administered food frequency questionnaire (FFQ) consisting of 75 food items was developed and calibrated against a four day weighed record (WR). The FFQ was also calibrated against a proxy for biomarker, the energy intake/basal metabolic rate ratio (EI/BMR). A total of 58 subjects (35 women, 23 men) aged between 19 and 76 years of Pakistani and Indian origin in Southampton, United Kingdom (UK) participated in this calibration study. The results for men and women combined together suggested reasonable agreement between FFQ and WR estimates of intake with Spearman rank correlation coefficient ranging between 0.26 and 0.38 (energy unadjusted). Gender specific agreement was however, poor. The percent mean difference between nutrient estimates by FFQ and WR was in general within 10% for energy, fat and protein in women while in the range of 11% to 25% in men. The percent mean difference for other nutrients such as sugar and dietary fibre ranged between 18% and 99% in men and women; while was 9% and 6% for starch in men and women respectively. The ranking of subjects into the thirds of distribution was poor with gross misclassification in the range of 5% to 14% in men and 15% to 29% in women. Comparison of both FFQ and WR with EI/BMR ratio showed that there were more underreporters of energy intake when reporting intake using WR than FFQ in both men and women. Based on these results, it was concluded that further development of the FFQ was needed before the FFQ could be used to evaluate the usual dietary intake in the South Asian population.
Introduction: The refeeding programme in government hospitals is aimed at
improving the nutritional status of malnourished children with weight-for-height
z-score (WHZ) below -2.0, using special therapeutic food. However, there is a lack of
data on the nutritional status of indigenous (Orang Asli) children when they return
to the community after hospital discharge. Case presentation: A 3-year-old Temiar
boy residing in a tribal village in Hulu Perak was visited to determine his nutritional
status following discharge from a refeeding programme in a government hospital.
He was admitted to the hospital with a weight of 10.0 kg, height of 85.5 cm and
WHZ of -2.09. The boy was later discharged weighing 11.0 kg, and with height
unchanged at 85.5 cm and WHZ of -0.87. During our visit to the child’s home
three months after discharge, his weight was 9.5 kg, height 86.0 cm, and WHZ
–2.91. Discussion: The management of the case in the hospital was based on
the Malnourished Orang Asli Protocols of the hospital. Household food insecurity,
feeding and care practices, unhealthy household environment and the lack of
communication between hospital and community health services were all identified
as risk factors for malnutrition. Conclusion: The recurrence of malnutrition in this
child, after successful improvement during hospital stay, highlights the importance
of identifying factors that may affect nutritional status after hospital discharge. This
knowledge will be beneficial in planning specific interventions, especially for Orang
Asli children, living in remote villages.
A study was carried out on 117 schoolchildren comprising 51 boys and 66 girls between the ages of 10 and 13 years to evaluate the effect of Ramadan-fasting on body weight. The results revealed that more than 90% of the subjects experienced a decrease in body weight during Ramadan. The initial mean body weight of the boys and girls were 35.7 ± 6.7 kg and 35.2 ± 6.1 kg respectively. After four weeks' fast, the boys and girls significantly lost (p<0.001) an average of 4.2% and 3.7% of their initial body weight respectively. Dietary intake and activity pattern were also assessed in a subgroup of 20 boys and 30 girls during the same period. The mean daily energy intake during Ramadan-fasting {boys 1230 ± 363 kcal (5.15 ± 1.52 MJ), girls 1034 ± 290 kcal (4.33 ± 1.21 MJ)} was significantly lower (p<0.001) than intake assessed before Ramadan {boys 1520 ± 463 kcal (6.36 ± 1.94 MJ), girls 1344 ± 428 kcal (5.62 ± 1.79 MJ)}. Both values were found to be below the suggested daily dietary intake for Malaysians of similar sex and age group recommended by Teoh (1975). Both boys and girls significantly decreased their intakes of fat, carbohydrate and thiamine during Ramadan. However, intakes of protein, niacin, vitamins A and C were found to be comparable to non-fasting values. During Ramadan, the mean intake of all nutrients analysed except protein were lower than recommended values. The boys spent significantly more (p<0.05) time sitting and significantly less (p<0.05) time standing and in moderately active pursuits during Ramadan. During this period, the boys spent more time in prayers compared with the girls. Time spent on all other activities did not differ between the fasting and non-fasting months. The findings suggest that the decrease in body weight during Ramadan may be primarily due to the reduction in energy intake since activity pattern remain fairly constant. The nutrient intake pattern of the adolescents studied may be of great concern in the long-term, as it does not meet the requirements for the normal growth of adolescents.
Introduction: Monitoring changes in energy and nutrient intakes of the population
over the course of time is essential to help healthcare providers develop effective
dietary policies. The aim of this study was to assess the changes in the nutrient
intake and Recommended Nutrient Intake (RNI) achievements by using the data
obtained from the Malaysian Adult Nutrition Surveys (MANS) that were carried out
in 2003 and 2014. Mis-reporting of energy intake was taken into account. Methods:
Dietary data were obtained from MANS 2003 and MANS 2014, which involved a
combined total of 4,044 randomly selected respondents, aged 18-59 years, using
a single 24-hour diet recall. Energy and nutrients calculations were based on the
Malaysian Food Composition database using the Nutritionist Pro software. The
results were compared against the RNI for Malaysia to assess dietary adequacy.
Results: The proportions of calories derived from macronutrients were within the
recommendations for a healthy diet. The consumption of protein, fat, calcium, iron
and vitamin A was significantly higher in 2014 than in 2003. The consumption
of protein, iron, vitamin C, and vitamin A was found to exceed the RNIs in 2014.
However, carbohydrate and sodium intakes had significantly decreased. Despite the
decrease, sodium intake still exceeded RNI recommendations. Conclusion: Signs
of changing energy and nutrient intakes were found, including increases in protein
and fat intakes since 2003, and decreased carbohydrates. This could be an alarming
indicator of the tendency to eat energy dense food among the population.
This quasi-experimental study was carried out to evaluate the effectiveness of an eight-week multi-disciplinary pulmonary rehabilitation programme in improving nutritional and functional status and quality of life of COPD out patients at Hospital Universiti Kebangsaan Malaysia. A total of 9 COPD outpatients aged 40 years and above (6 men and 3 women) completed at least 50% of 16 sessions of an eight-week pulmonary rehabilitation programme. Their nutritional and functional status and rating of quality of life were measured at baseline (0 day) and after 8 weeks and these were compared to those of the control group matched for age, comprising 13 subjects (11 men and 2 women). Nutritional status was determined using anthropometry, body composition (Bioelectrical Impedance Analysis) and three-day food record. Assessment of appetite for food was also carried out using the Simplified Nutritional Assessment Questionnaire (SNAQ). Functional status was assessed using the Pulmonary Functional Status and Dyspnea Questionnaire-modified version(PFSDQ-M). The handgrip strength was also measured using handgrip dynamometer. The SF36 questionnaire was used to measure the quality of life of the subjects. There was a reduction in dyspnea (-49.0%, p<0.05) and fatigue (-47.8%, p<0.05) in men after the intervention programme, as compared to their controls (dyspnea -2.9% and fatigue 8.9%). Quality of life in this group was also significantly improved by 31.8% (p<0.05) as compared to their controls (-3.0%,p>0.05). Similar trends were noted for the women, although the difference was not significant. No significant changes were seen in nutritional status assessed by anthropometry, dietary intake and appetite. However, there was a trend of increased nutrient intake and SNAQ score in the intervention group as compared to control. An eight-week pulmonary rehabilitation programme was effective in improving the functional status particularly in men, by reducing dyspnea and fatigue; and also improving their quality of life, but was not effective in changing the nutritional status.
Study site: Physiotherapy Unit, Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM), Kuala Lumpur, Malaysia
The antimicrobial activity of the Centipeda minima L. (Asteraceae) extract was evaluated against seven microorganisms using the disc diffusion method. The extract showed a broad spectrum of antimicrobial activity against all the tested bacterial strains, especially Enterobacter aerogenes, Klebsiella pneumonia, Staphylococcus aureus and Yersinia enterocolitica. The chemical composition of the extract obtained from Centipeda minima was analysed by GC-MS. Twentythree compounds, constituting about 84.75 % of the total extract, were identified. The main constituents were palmitic acid (7.47%), (Z,Z)-9-,12-octadecatrienoic acid (6.52%), (Z,Z,Z)-9-,12-octadecatrienoic acid (7.01%), phytol (7.01%), naptho[2.3-b]furan-2-(3H)-on (6.21%), 1-(1,2,3,4,7,7a-hexahydro-1,4,4,5-tetramethyl-1,3a-ethano-3aH-inden-6-yl)etanon (7.95%), 1,3,5-tri-tertbutyl- benzene (4.52%), (3Z)-2-methyl-3-octen-2-ol (5.95%) and artemisia ketone (4.98%). The extract was also tested against brine shrimp for toxicity. There was no significant toxicity as it only recorded a LC50 value of 4.92 mg/ml. The study shows that the extract is a good antimicrobial agent with potential applications in public health against diseases.