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  1. Haque MA, Rafii MY, Yusoff MM, Ali NS, Yusuff O, Arolu F, et al.
    Mol Biol Rep, 2023 Mar;50(3):2795-2812.
    PMID: 36592290 DOI: 10.1007/s11033-022-07853-9
    Natural and man-made ecosystems worldwide are subjected to flooding, which is a form of environmental stress. Genetic variability in the plant response to flooding involves variations in metabolism, architecture, and elongation development that are related with a low oxygen escape strategy and an opposing quiescence scheme that enables prolonged submergence endurance. Flooding is typically associated with a decrease in O2 in the cells, which is especially severe when photosynthesis is absent or limited, leading to significant annual yield losses globally. Over the past two decades, considerable advancements have been made in understanding of mechanisms of rice adaptation and tolerance to flooding/submergence. The mapping and identification of Sub1 QTL have led to the development of marker-assisted selection (MAS) breeding approach to improve flooding-tolerant rice varieties in submergence-prone ecosystems. The Sub1 incorporated in rice varieties showed tolerance during flash flood, but not during stagnant conditions. Hence, gene pyramiding techniques can be applied to combine/stack multiple resistant genes for developing flood-resilient rice varieties for different types of flooding stresses. This review contains an update on the latest advances in understanding the molecular mechanisms, metabolic adaptions, and genetic factors governing rice flooding tolerance. A better understanding of molecular genetics and adaptation mechanisms that enhance flood-tolerant varieties under different flooding regimes was also discussed.
  2. Oladosu Y, Rafii MY, Arolu F, Chukwu SC, Salisu MA, Olaniyan BA, et al.
    Plants (Basel), 2021 Aug 20;10(8).
    PMID: 34451758 DOI: 10.3390/plants10081714
    Eggplant is the fifth economically most important vegetable in the Solanaceae family after tomato, potato, chili, and tobacco. Apart from the well-cultivated brinjal or aubergine eggplant (Solanum melongena L.), two other underutilized eggplant species, the African eggplant (S. macrocarpon L.) and the scarlet eggplant (S. aethiopicum L.), were also cultivated with local importance where the leaves and fruits are used for food and medicinal purposes. The major objectives of the eggplant breeding program are to improve fruit quality, increase yield performance through heterosis breeding, and introduce pest and disease resistances from wild relatives. Europe and Asia hold a wide collection of germplasm resources with significant potential for genetic improvement. While cultivated eggplant is susceptible to several fungi and bacteria, many wild relatives offer potential resistance to these pathogens. In this paper, we review the genetic resources and diversity of cultivated eggplant and its wild relatives. As a point of departure, we examine the economic importance, domestication, taxonomy characterization, and relationships of the crop and its wild relatives. The importance of evaluating and safeguarding wild relatives is highlighted, as crop wild relatives are highly underrepresented. A key section in this study is an overview dedicated to genetic resources, resistance to biotic and abiotic stresses, pre-breeding, and breeding for sustainable eggplant production.
  3. Hashim A, Rafii MY, Yusuff O, Harun AR, Juraimi S, Misran A, et al.
    Heliyon, 2024 Feb 29;10(4):e25111.
    PMID: 38370252 DOI: 10.1016/j.heliyon.2024.e25111
    Induced mutation for the creation of desirable traits through chronic gamma irradiation provides an opportunity for the selection and development of new chili varieties. This study was conducted to assess the effects of different doses of chronic gamma irradiation on morpho-physiological traits in chili. Ten plants from each variety were exposed to different doses of chronic gamma irradiation for 277.02 h at three weeks after germination under gamma greenhouse facilities, with accumulative dose; 185.61Gy, 83.11Gy, 47.096Gy, 30.474Gy, 19.4Gy, 13.9Gy, 11.1Gy, 8.31Gy, 5.54Gy) and 2.77Gy respectively. Highly significant differences were observed among doses (Rings) of chronic gamma irradiation expressed in mean values for all investigated traits. Relatively moderate doses of chronic gamma irradiation represented by doses 47.096 Gy (Ring 4) and 19.40 Gy (Ring 6) resulted in significant stimulation for most of the studied characters. The highest heritability was recorded in days to flowering at 99.88 while the lowest was observed in fruit dry weight at 34.66 %. High genetic advance were recorded for most of the quantitative traits studied. In addition, a highly significant positive correlation was observed between total fruit per plant, total number of fruit per plant, plant height, fruit fresh weight, number of secondary branches, chlorophyll a, fruit dry weight, total chlorophyll content, stem diameter, fruit length and fruit girth. With increasing chronic gamma dose, mutagenic efficiency and efficacy generally increased. Induced variety of desirable features will considerably increase the chilli's amelioration through mutation breeding, leading to the development of improved varieties. The results of this research offer valuable information for the use of chronic gamma radiation in the mutations breeding of Capsicum annuum L., which will be advantageous for future breeding programs.
  4. Myint KA, Yaakub Z, Rafii MY, Oladosu Y, Samad MYA, Ramlee SI, et al.
    Biomed Res Int, 2021;2021:6620645.
    PMID: 33997027 DOI: 10.1155/2021/6620645
    Molecular characterization of oil palm germplasm is crucial in utilizing and conserving germplasm with promising traits. This study was conducted to evaluate the genetic diversity structures and relationships among 26 families of MPOB-Senegal oil palm germplasm using thirty-five microsatellite markers. High level of polymorphism (P = 96.26%), number of effective allele (N e = 2.653), observed heterozygosity (H o = 0.584), expected heterozygosity (H e = 0.550), total heterozygosity (H T = 0.666), and rare alleles (54) were observed which indicates that MPOB-Senegal germplasm has a broad genetic variation. Among the SSR markers, sMo00053 and sMg00133 were the most informative markers for discrimination among the MPOB-Senegal oil palm germplasm for having the highest private alleles and the rare alleles. For selection and conservation, oil palm populations with high rare alleles and Nei's gene diversity index should be considered as these populations may possess unique genes for further exploitation.
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