Fungi are ubiquitous eukaryotic microorganisms that are often implicated in a wide range of diseases. Exposure to fungal bioaerosols is often associated with conditions such as asthma, allergic rhinitis, keratitis, and pneumonitis, among others. This study aims to shed some light on the configuration of airborne culturable fungal genera in the atmosphere of Kuwait City with analysis of seasonal patterns, intra-diurnal variations and correlations with meteorological factors. Outdoor fungal bioaerosols samples were collected twice a day, two times a week, from January 2016 to December 2023 via volumetric active sampling in Al-Sabah Medical District, Kuwait City, Kuwait. The meteorological data were supplied by Kuwait Meteorological Department, Directorate General of Civil Aviation. The rates of fungal bioaerosols remained relatively high during the months of March, April and May, ranging from 269 to 296 CFU/m3. Cladosporium, Aspergillus and Alternaria were the most common fungal genera in the atmosphere, with a prevalence of 49%, 16% and 14%, respectively. Statistically significant differences (p