Cocos nucifera (L.) belongs to Arecaceae family is a type of highly valuable plant species due to its medicinal values. It is a widespread fruit at the area of Southeast Asia and commonly known as “coconut tree”. The fruit part of Cocos nucifera (L.) contains coconut water that have many pharmacological activities which can give benefits for human health and also give benefits for plant in tissue culture Therefore, coconut water has been associated very well in various tribes around the world with diverse biological effects for human such as anti-inflammatory, antifungal, antioxidant, renal protective and cardio protective activity. Besides that, coconut water was noted for its wide application in plant tissue culture due to its nutrients content. It was reported to be served as the organic additives for plant to enhance callus induction and proliferation, shoot and root elongation/regeneration and somatic embryogenesis in tissue culture. The present article will discuss the pharmacological activities of coconut water as well as its benefits for plant in tissue culture which had been proven based on recent scientific research.
This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA was tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5-2.0 mg/L BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%).