Membrane distillation (MD) is a thermally driven technology applied in desalination and water reuse with utilisation of sustainable energy. However, algal organic matter (AOM) could foul membrane critically and plague MD's long-term operational stability. In this study, the soluble extracellular polymeric substance (sEPS) and intracellular organic matter with bound extracellular polymeric substance (IOM + bEPS) of two algal species (Amphora coffeaeformis and Navicula incerta) were exposed to 60 °C, 70 °C and 80 °C for 8 h with polypropylene hydrophobic membrane, simulating heated AOMs contacted with membrane inside MD unit, to study the temperature effect on membrane fouling. The dissolved carbohydrate and protein in the sEPS and IOM + bEPS samples generally increased after being heated. Heating caused cell lysis and the release and dissolution of carbohydrate and protein from sEPS, IOM and bEPS into water. As heating temperature increased, the carbohydrate release from the AOM usually increased. The contact angle of membrane contacted with sEPS and IOM + bEPS reduced significantly after heat treatment. The reduction in IOM + bEPS was larger than sEPS, in line with SEM analysis, indicating membrane surfaces and pores with IOM + bEPS fouled more severely than sEPS. It is due to higher hydrophobicity in IOM + bEPS causing adherence to membrane and presence of amphiphiles. High protein, lipid, and saturated fats proportions also cause severe fouling. SEM-EDX analysis indicated presence of O, Na, Cl and Mg elements, pointing to carbohydrate and lipids, and salt trapped in foulants. AOM heating and composition had direct effect to the membrane integrity, dictating severity of fouling in MD operations.
An artificial magnetotactic microbot was created by integrating the microalgal cell with magnetic microbead for its potential application as biomotor in microscale environment. Here, we demonstrate the remote magnetotactic control of the microbot under a low gradient magnetic field (<100 T/m). We characterize the kinematic behavior of the microbots carrying magnetic microbeads of two different sizes, with diameter of 2 and 4.5 μm, in the absence and presence of magnetic field. In the absence of magnetic field, we observed the microbot showed a helical motion as a result of the misalignment between the thrust force and the symmetry axis after the attachment. The microbot bound with a larger magnetic microbead moved with higher translational velocity but rotated slower about its axis of rotation. The viscous force was balanced by the thrust force of the microbot, resulting in a randomized swimming behavior of the microbot at its terminal velocity. Meanwhile, under the influence of a low gradient magnetic field, we demonstrated that the directional control of the microbot was based on following principles: (1) magnetophoretic force was insignificant on influencing its perpendicular motion and (2) its parallel motion was dependent on both self-swimming and magnetophoresis, in which this cooperative effect was a function of separation distance from the magnet. As the microbot approached the magnet, the magnetophoretic force suppressed its self-swimming behavior, leading to a positive magnetotaxis of the microbot toward the source of magnetic field. Our experimental results and kinematic analysis revealed the contribution of mass density variation of particle-and-cell system on influencing its dynamical behavior.