The aim of this study was to describe and systematically evaluate the psychometric properties of attitudes to aging measures that have been validated in adults younger than 60 years. Studies were assessed for methodological quality using the COnsensus-based Standards for the selection of health Measurement INstruments. Best evidence synthesis was performed to summarize the levels of evidence. Systematic searching yielded 21 articles, relating to 10 different measures. Some instruments were validated only in middle-aged and older people, while others were validated in a wider age range. Measures differed in whether their items related to participants' own experiences of aging, their anticipated future aging, and/or aging in general. None of the measures had had all of its psychometric properties assessed. The Expectations Regarding Ageing-12 and the Anxiety About Ageing Scale received positive ratings for the greatest number of psychometric properties, but capture different constructs, and may be differentially suited to different age groups of younger adults.
Objectives: More positive attitudes to aging are associated with better health and wellbeing outcomes. This study examined whether chronological age moderated relationships between attitudes to aging and wellbeing outcomes, and whether these relationships differ according to the specific attitudinal construct measured.Method: Participants were 911 adults aged 18-60 years (mean = 36.63 years). Attitudes to ageing were measured using the Malay Reactions to Ageing Questionnaire (M-RAQ), which focuses on respondents' anticipated reactions to being aged over 65 years, and the Malay Anxiety about Ageing Scale (M-AAS), which additionally captures respondents' fears and anxieties about current aging and older people. Wellbeing outcomes were measured using the Warwick- Edinburgh Mental Well-being Scale (WEMWBS) and the Satisfaction with Life Scale (SWLS).Results: More positive attitudes to ageing were associated with higher levels of wellbeing and life satisfaction after controlling for age, gender, education, and self-rated health. Age moderated the relationships between the M-AAS and wellbeing and life satisfaction, with stronger relationships in younger adults. No moderating effects of age were found for the M-RAQ.Conclusion: The difference in the moderating effects of age between measures suggests that the two attitudinal constructs captured by these two measures operate in different ways. In particular, the current emotional/anxiety components of attitudes to ageing may differentially affect wellbeing at different ages. This suggests that interventions aimed at the promotion of wellbeing through coping with aging-related anxieties might be particularly well-suited to younger adults, who may be less able to respond effectively to the anxieties they experience.
The aims of this study were to culturally adapt the Reactions to Ageing Questionnaire (RAQ) for a non-Western (Malay) population, and explore attitudes to ageing in relation to age, gender, and education. Eight new culturally relevant items were generated by asking Malay-speaking participants about their reactions to ageing. A Malay version of the extended 35-item RAQ was then administered to 911 Malaysian participants aged 18 to 60 years. Exploratory factor analysis revealed four factors. Three of the factors were similar to those identified in the original RAQ, while "Family and Religion" emerged as a new factor. More negative attitudes to ageing were observed in younger and female respondents. There were no effects of education. This culturally adapted RAQ exhibits robust psychometric properties, and could be used to assess attitudes to ageing in Malaysia and other Southeast Asian countries. Moreover, we have identified a "core set" of RAQ items that could be applicable worldwide.
A portable electrochemical aptamer-antibody based sandwich biosensor has been designed and successfully developed using an aptamer bioreceptor immobilized onto a screen-printed electrode surface for Mycobacterium tuberculosis (M. tuberculosis) detection in clinical sputum samples. In the sensing strategy, a CFP10-ESAT6 binding aptamer was immobilized onto a graphene/polyaniline (GP/PANI)-modified gold working electrode by covalent binding via glutaraldehyde linkage. Upon interaction with the CFP10-ESAT6 antigen target, the aptamer will capture the target where the nano-labelled Fe3O4/Au MNPs conjugated antibody is used to complete the sandwich format and enhance the signal produced from the aptamer-antigen interaction. Using this strategy, the detection of CFP10-ESAT6 antigen was conducted in the concentration range of 5 to 500 ng/mL. From the analysis, the detection limit was found to be 1.5 ng/mL, thereby demonstrating the efficiency of the aptamer as a bioreceptor. The specificity study was carried out using bovine serum albumin (BSA), MPT64, and human serum, and the result demonstrated good specificity that is 7% higher than the antibody-antigen interaction reported in a previous study. The fabricated aptasensor for M. tuberculosis analysis shows good reproducibility with an relative standard deviation (RSD) of 2.5%. Further analysis of M. tuberculosis in sputum samples have shown good correlation with the culture method with 100% specificity and sensitivity, thus making the aptasensor a promising candidate for M. tuberculosis detection considering its high specificity and sensitivity with clinical samples.