Nisamalaki Churna is an Ayurveda formulation used for diabetes, which consists of amalaki and haridra. in vitro antidiabetic screening of Nisamalaki Churna by α-amylase inhibition was carried out by starch iodine method, dinitrosalicylic acid method (DNSA) and effect on normal rats blood sugar level was studied. Nisamalaki Churna shows potent α-amylase inhibition IC50 89.44 µg/mL by starch iodine method and IC50100.0 µg/mL by DNS method. Nisamalaki Churna showed potent decrease in blood glucose level in normal rats.