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Comparative pathogenicity of Malaysian variant and very virulent infectious bursal disease viruses in chickens

Aliyu HB, Hamisu TM, Hair Bejo M, Omar AR, Ideris A

Avian Pathol, 2022 Feb;51(1):76-86.
PMID: 34842475 DOI: 10.1080/03079457.2021.2006604

Variant infectious bursal disease virus (vaIBDV) has been identified in various countries with significant economic losses. Recently, the first identification of a variant strain in Malaysia was reported. The pathogenicities of the Malaysian variant, UPM1432/2019, and very virulent infectious bursal disease virus (vvIBDV), UPM1056/2018 strains were comparatively evaluated in specific-pathogen-free (SPF) chickens based on gross and histopathological examinations and viral load. Four-week-old SPF chickens were randomly divided into three groups; group 1 served as the control, while groups 2 and 3 birds were challenged with the vaIBDV and vvIBDV, respectively. Three birds from each group were weighed, euthanized and necropsied at 2, 3, 4, 5, 7 and 21 days post-challenge (dpc). Unlike UPM1056/2018 group, birds from UPM1432/2019 group did not show clinical signs or death. UPM1056/2018 strain caused 11% mortality rate in the infected chickens. The bursal body index (BBIX) for UPM1432/2019- and UPM1056/2018-infected groups was <0.7 from 2 dpc and continued to decrease to 0.49 and 0.45, respectively, at 21 dpc. UPM1432/2019 strain was more persistent in the bursa than UPM1056/2018 strain. Both strains induced similar pathological lesions in SPF chicks. These results indicate that the Malaysian vaIBDV severely damaged the immune organs of chickens and was more persistent in bursal tissue than vvIBDV. The study provides insight into the pathogenicity of the variant strain as further study may be required to evaluate the efficacy of the currently available IBD vaccines in Malaysia against the strain. RESEARCH HIGHLIGHTSEmerging Malaysian variant IBDV caused severe bursal damage without mortality.Atypical vvIBDV induced bursal atrophy with inflammatory response and caused low mortality.Malaysian variant IBDV was more persistent in bursal tissue than vvIBDV.
Fulltext A retrospective study of bovine tuberculosis at the municipal abattoir of Bauchi State, Northeastern Nigeria

Jajere SM, Atsanda NN, Bitrus AA, Hamisu TM, Goni MD

Vet World, 2018 May;11(5):598-605.
PMID: 29915497 DOI: 10.14202/vetworld.2018.598-605

Background and Aim: Bovine tuberculosis (bTB) still remains a major zoonotic bacterial disease affecting livestock and humans worldwide. The disease remains a poorly managed tropical disease in most developing countries of the world; where in addition to productivity losses and significance in international trade, it posed a major public health threat to both humans and animals. A retrospective study was designed to investigate the occurrence of bTB lesions at Bauchi municipal abattoir.
Materials and Methods: The study utilized abattoir records spanning a period of 10 years (2004-2013). The records indicated that a total of 1,08,638 heads of cattle comprising n = 56,070 males and n = 52,570 females were slaughtered at the municipal abattoir during the study period.
Result: Of these heads, n = 1230 (1.13%) (95% confidence interval [CI]: 1.07, 1.19) had tuberculous lesions. The annual occurrence during the study period varied significantly (p<0.001) from 0.53% (95% CI: 0.40, 0.67) to 1.87% (95% CI: 1.66, 2.10) in 2010 and 2012, respectively. Females had a significantly higher (p<0.001) prevalence of 2.10% (95% CI: 1.98, 2.23) compared with the males 0.23% (95% CI: 0.19, 0.27). The distribution of suspected gross bTB lesions in different organs showed 11.87% in the lungs, 5.93% in the liver, 1.14% in the heart, and 0.49% accounted for generalized bTB. However, none was observed on the lymph nodes and intestines.
Conclusion: It can be concluded that bTB persists in Bauchi State with annual variations during the study period. This study highlights the importance of meat inspection as an important tool for detecting the presence of bTB lesions.
Fulltext Prevalence and burden of gastrointestinal helminthes among grey-breasted helmet guinea fowls (Numida meleagris galeata) encountered in Gombe state, Nigeria

Jajere SM, Lawal JR, Atsanda NN, Hamisu TM, Goni MD

Int J Vet Sci Med, 2018 Jun;6(1):73-79.
PMID: 30255082 DOI: 10.1016/j.ijvsm.2018.04.007

This cross sectional survey was conducted from July to December 2015 in order to investigate the burden of gastrointestinal helminthes among guinea fowls in Gombe, Northeastern Nigeria. A total of six hundred fowls (viscera) were purchased from six randomly selected slaughter slabs. Out of the 600 birds examined, 479 (79.83%; 95% CI: 76.4, 82.9) were found harbouring one or more gastrointestinal helminthes. Of this, 238 birds (39.7%; 35.8, 43.6) were infected by nematode species and 392 birds (65.3%; 61.4, 69.0) by cestode species. A total of nine nematodes and seven cestodes species were recovered from these birds. There was no any trematode observed among the studied birds. The prevalences of the nematodes identified in descending order were: Ascaridia galli 56.7% (52.7, 60.6); Ascaridia numidae 38.0% (34.2, 42.0); Heterakis gallinarum 17.2% (14.4, 20.4); Heterakis meleagridis 8.3% (6.4, 10.8); Strongyloides avium 3.5% (2.3, 5.3); Subulura brumpti 3.2% (2.0, 5.0); Gongylonema ingluvicola 2.2% (1.3, 3.7) and both Dispharynx spiralis and Tetrameres numidae had 0.7% (0.3, 1.7). While for cestodes: Raillietina tetragona 72.8% (69.1, 76.2); Raillietina echinobothrida 67.3% (63.5, 71.0); Raillietina cesticillus 50% (46.0, 54.0); Raillietina magninumida 25.7% (22.3, 29.3); Hymenolopsis cantaniana 17.3% (14.5, 20.6); Davainea nana 4.2% (2.8, 6.1) and the lowest was observed in Choanotaenia infundibulum with 2% (1.2, 3.5). Infection rates did not differ significantly based on sex (P > 0.05). However, the occurrence of mixed infection as compared with single infection was statistically significant in both cestodes and nematodes (P 
Expression Profiles of Immune-Related Genes and Apoptosis Study of Avian Intraepithelial-Natural Killer Cells in Chickens Inoculated with Vaccine Strain of Newcastle Disease Virus (NDV) and Challenged with Virulent NDV

Hamisu TM, Aliyu HB, Tan SW, Hair-Bejo M, Omar AR, Ideris A

Avian Dis, 2022 Sep 22.
PMID: 36198006 DOI: 10.1637/aviandiseases-D-22-00029

In spite of the available information on the role of natural killer (NK) cells in several viral infections, the interactions between chicken intraepithelial-NK (IEL-NK) cells and Newcastle disease virus (NDV) are poorly understood. In this study, we investigated these interactions following the inoculation of chickens with NDV vaccine strain LaSota and subsequent challenge with velogenic NDV (vNDV) genotype VII (GVII) and VIII (GVIII), through quantification of IEL-NK cell's apoptosis and expression profiling of its surface receptors. Specific-pathogen-free chickens were randomly divided into six groups, as follows: one group of an uninfected control, one group infected with NDV LaSota, two groups each infected with either GVII or GVIII, and two groups inoculated with NDV LaSota and challenged with either GVII (LaSota-genotype VII [LSGVII]) or GVIII (LaSota-genotype VIII [LSGVIII]). Avian intraepithelial lymphocytes (IEL) were isolated from the duodenal loops, and CD3- cells were characterized. Immunophenotyping and apoptosis analysis of CD3-/CD25+/CD45+IEL NK cells were conducted using a flow cytometer. In addition, a gene expression study was conducted using real-time quantitative PCR. Data were analyzed using two-way analysis of variance. The results showed that vNDV GVII or GVIII caused apoptosis of IEL-NK cells; however, following inoculation of LSGVII or LSGVIII, the effect of vNDV GVII and GVIII to cause a reduction in the population of viable IEL-NK cells was significantly reduced. Furthermore, the expression profiles of activating receptors CD69, NK-lysin, and IFN-γ, were generally upregulated in chickens inoculated with LSGVII or LSGVIII. In contrast, B-NK, an inhibitory receptor, was downregulated in these treatment groups. In NDV GVII- and GVIII-challenged groups, however, B-NK was upregulated, whereas the other receptors were generally downregulated. The findings of this study showed that NDV vaccine strain LaSota may prevent apoptosis and cause upregulation of activating receptors of chicken IEL-NK cells in velogenic virus-challenged settings.