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  1. Fulltext Whole genome resequencing data and grain quality traits of the rice cultivar Mahsuri and its blast disease resistant mutant line, Mahsuri Mutant
    Kamarudin SAA, Ahmad F, Hasan N, Hisham SN, Yusof SN, Abu Hassan A, et al.
    Data Brief, 2024 Feb;52:109974.
    PMID: 38229927 DOI: 10.1016/j.dib.2023.109974
    In Malaysia, rice mutant varieties that are genetically altered to confer resistance against blast disease have been substantially developed through mutational breeding program. However, due to the limited accessible information on the mutant lines, mutant gene variants corresponding to the disease resistance and other useful agronomic traits are yet to be exploited. Here, we conducted whole genome re-sequencing of blast resistance with kernel elongation traits in mutant line, Mahsuri Mutant (87,639,446 bp raw reads), and its parental line, Mahsuri (85,156,783 bp raw reads) using Illumina Novaseq 6000 sequencing platform with 30x sequencing coverage. The generated genome sequences are aimed to facilitate the discovery of causal mutation and single nucleotide polymorphisms (SNPs) related to the intended traits. The identified SNPs can be further employed to develop allele-specific SNP molecular markers to locate the mutant gene regions. The NGS data obtained (FASTQ format) of the parental and mutant lines have been deposited in the National Center for Biotechnology Information (NCBI) database under sequence read archive (SRA) xwith accession numbers SRR24388814 (Mahsuri) and SRR22952097 (Mahsuri Mutant) respectively.
  2. Fulltext Outbreak of foodborne disease in a boarding school, Negeri Sembilan state, Malaysia, 2021
    Bujang NNA, Wahil MSA, Abas SA, Amin KHAK, Zulkifli NI, Shah SM, et al.
    Western Pac Surveill Response J, 2023;14(3):1-7.
    PMID: 37955032 DOI: 10.5365/wpsar.2023.1.4.3.1043
    OBJECTIVE: Foodborne disease is a significant global public health concern, with Bacillus cereus being a frequent cause of outbreaks. However, due to the relatively mild symptoms caused by infection with B. cereus, the shorter duration of illness and the challenges of testing for it in both stool and food samples, outbreaks are often underreported. This report describes the epidemiology of cases of foodborne illness, the causative agent and risk factors associated with an outbreak in a boarding school in Seremban district, Negeri Sembilan state, Malaysia, that occurred in November 2021.

    METHODS: Epidemiological, environmental and laboratory investigations were performed. A case was defined as any person with abdominal pain, vomiting or diarrhoea that occurred after consuming food served by the canteen at the school. The data were analysed using Microsoft Excel and the Statistical Package for the Social Sciences (SPSS).

    RESULTS: A total of 152 cases were identified among the 597 students, giving an attack rate of 25.5%. All cases were females aged 13-17 years. They presented with abdominal pain (100%), nausea (97.4%, 148), vomiting (78.3%, 119) or diarrhoea (61.8%, 94), or a combination of these. The mode of transmission of the outbreak was a continual common source. The foods associated with becoming a case were beef rendang (a dry curry) (odds ratio [OR]: 20.54, 95% CI: 4.89-86.30), rice (OR: 19.62, 95% CI: 2.62-147.01), rice cubes (OR: 18.17, 95% CI: 4.31-76.55) and vermicelli (OR: 17.02, 95% CI: 4.03-71.86). Cross-contamination and inadequate thawing and storage temperatures contributed to the outbreak.

    DISCUSSION: This outbreak of foodborne illness at a boarding school was likely caused by B. cereus. The findings highlight the importance of proper food preparation, temperature monitoring, hygiene practices among food handlers and compliance with food safety guidelines.

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