Introduction: In Malaysia, private office workers tend to be more physically inactive due to work burden. This study aimed to determine physical activity level, motivation factors and barrier towards physical activity among private office workers. Methods: A cross sectional study using convenience sampling was conducted at nine private com- panies in Selangor involving 106 office workers aged 21 to 55 years old. Self-administered questionnaires includ- ing Global Physical Activity Questionnaire (GPAQ), Physical Activity and Leisure Motivation Scale (PALMS) and Physical Activity Barrier (PAB) were administered. Results: Over half of the respondents (58.5%) were having low physical activity level. The main motivation factors include physical condition, psychological condition and mastery. Meanwhile, tired after work, laziness, lack of discipline, family commitment and adverse weather were the main perceived barriers. A weak positive correlation was found between total motivation score and total physical activity (r=0.296; p=0.002). In contrast, there was a weak negative correlation between barriers and total physical activity (r=-0.237; p=0.015). Conclusion: The current physical activity level, its main motivation factors and barriers among private office workers were identified, providing an opportunity to create effective setting-based health promotion and intervention. It is also recommended that policy suggestions are made to influence and prepare essential partners (e.g. companies and employees) to promote ways of incorporating physical activity into one’s daily routine.