Spore-forming bacteria, Bacillus sp., frequently been associated with the contamination of rice and other starchy
products. Spores are more resistant to antimicrobial treatments than its vegetative cells. The extract of Indonesian bay
leaf (Syzygium polyanthum L.) was assessed for its antibacterial and sporicidal activities against vegetative cells and
spores of B. cereus isolated from rice (25 strains). The results showed that S. polyanthum L. extract was able to inhibit
the growth of vegetative cells of all B. cereus isolates with MICs ranged from 0.16 to 0.63 mg/mL and can kill with
MBCs ranged from 0.31 to 2.50 mg/mL. The bactericidal endpoint for B. cereus BC-NP.8 in time kill curve was at 1.25
mg/mL (8× MIC) after 4 h of incubation while for B. cereus ATCC 33019 was at 2.50 mg/mL (8× MIC). The sporicidal
activity of S. polyanthum L. extract was not affected by different temperatures treatment and alteration of the pHs of
extract. Therefore, this indicates that the extract was stable after exposed to pH3, 7 and 10 as well as temperature
of 50, 80, and 121°C. Observation under on scanning electron microscope the structure of the B. cereus ATCC 33019
spores was ruptured after being treated with 1% (w/v) S. polyanthum L. extract for 1 h. In conclusion, S. polyanthum L.
extract had antibacterial and sporicidal activity against vegetative cells and spores of B. cereus isolated from rice.