Fibroblast is one component of connective tissue cells in polygonal or stellate with cytoplasmic processes or projections. In vitro, tissue culture is an excellent medium used in biomedical research. The goal of this study was to analyze Toxicity and Malignancy in Embryo of Super Java Chicken as Potential Candidates of Strong Poultry in Indonesia. This study used Preparation of fibroblast primary cell culture of Java super chicken embryos, Pathogenicity test using fibroblast cells from Super Java chicken, Toxicity test using fibroblast cells from Super Java Chicken. Primary fibroblast cell culture of Java super chicken embryos was prepared from 10-day-old brood chicken eggs free of pathogens. Cells were prepared in 96-well microplates with minimal essential medium (MEM) containing 10 % fetal calf serum (FCS) 100 IU/ug penicillin/streptomycinVirus isolates were diluted in stages from 10-2 to 10-5 and inoculated into Java Super Chicken Fibroblast cell culture. The result showed that the negative control of the samples had a faster proliferative power than the fibroblast cell culture of Java super chicken, which was treated with concentrations of 10-2; 10-3; 10-4; 10-5. Moreover, before being inoculated with the virus, the confluent fibroblast cells of Java super chicken looked oval and regular. The day after infection, syncytia (large multinucleated cells) began to form on a small scale and became more pronounced on the second and third post-infection days. CPE was found in the 10-2,10-3 and 10-4 virus dilutions, and CPE was not found in the 10-5 dilution.
In veterinary medicine, the concept of disease is critical because it related to the survivability rate of the veterinary, especially livestock. Chicken was the most popular livestock that was observed in veterinary medicine. However, veterinary books were less popular compared to article and conference paper in global academic. This study's goal was to look how was the depiction of topic disease was used in veterinary textbooks that were related to the embryo of chicken as well as the trend of that topic. This study gathered 90 books meta-data donwloaded from Scopus website in form of CSV file. The data were analyzed using Vosviewer and biblioshiny of R Studio software to see the topic trend, citation, and number of book pages. Literature review also used to see the depiction of disease inside samples. Result showed that authors' keywords, heart and disease were closely related with a keyword chicken embryo. Moreover, each book get at least 10-11 citations globally. Moreover, repetitive keywords used in abstract of samples of this study were cells/cell, gene, and human. Those repetitive words were closely related to a word disease. It could be means that cell of the embryo of chicken also played the important role in determining its resistance against disease.