Yellowfin tuna (Thunnus albacares) wastes were used to recover of tuna frame (TF) and to extract of tuna bone powder (TBP) by alkaline treatment. The amount of the calcium with 24.56% and 38.16% was the most abundant element in TF and TBP, respectively. Nine essential amino acids for human body; lysine, valine, leucine, isoleucine, methionine, threonine, histidine, phenylalanine and tryptophan are detected in TF and TBP. The amount of the collagen associated amino acids such as glycine, proline and hydroxyproline were high and the amount of glutamic acid, arginine, alanine, aspartic acid and serine were relatively more. The amount of the oleic acid, palmitic acid and gondoic acid were higher and the amount of hexadecatrienoic acid, γ-linolenic acid and dihomo-γ-linolenic acid were lower. The amount of the myristic acid, stearic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were also relatively more. Results demonstrated that TBP as inexpensive and environmentally friendly alternative source of calcium can be converted to the healthy value added products to increase of the amount of the calcium intake among people especially population groups with lactose intolerance.