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Fulltext Lost and found! Tooth fragment reattachment after 8 Mo of trauma

Mani Kakkunath S, Katta NK, Shankar Yelamanchi R, Mandava D

Clin Case Rep, 2019 Nov;7(11):2271-2273.
PMID: 31788301 DOI: 10.1002/ccr3.2463

Thorough clinical and radiographic evaluation of patient following orofacial trauma is indispensable for a successful outcome, and when the tooth fragment is available in a good condition, then fragment reattachment is the best choice of treatment.
Fulltext Evaluation of Levels of Proinflammatory Chemokines MIP-1α and MIP-1β in Gingival Crevicular Fluid of Primary, Mixed and Permanent Dentition

Swathi B, Charitha M, Mandava D, Tugaram N, Mudrakola DP, Yelamanchi R

J Int Soc Prev Community Dent, 2019 04 12;9(2):205-209.
PMID: 31058072 DOI: 10.4103/jispcd.JISPCD_438_18

Aim: This study aims to find out the proinflammatory chemokines macrophage inflammatory protein (MIP)-1α and MIP-1β levels in gingival crevicular fluid (GCF) of primary, mixed, and permanent dentitions.
Materials and Methods: GCF of 160 individuals (4-15 years of age) was collected by the extracrevicular method. They were categorized into four groups (40 per each group). Group I: subjects with primary dentition (4-5 years of age), Group II: 40 subjects in early transition period (6-8 years), Group III: 40 individuals in the late transition period (9-11 years), and Group IV: 40 individuals with permanent dentition (12-15 years). MIP-lα and MIP-1β levels were determined in the samples of GCF by ELISA method. Data were analyzed by software SPSS Version 20 (IBM SPSS Statistics for Windows, IBM Corp., Armonk, NY: USA).
Results: MIP-1α and MIP-1β were detected in all samples. The highest mean MIP-1α and MIP-1β concentrations in GCF were detected in the early transition period, while the lowest concentrations were seen in primary dentition group. The chemokine levels were higher in girls than in boys in Group III. There was a substantial rise of MIP-1α and MIP-1β levels during eruption.
Conclusions: Since levels of MIP-1α and MIP-1β in GCF are positively associated with tooth eruption, they may perhaps be deemed as novel biomarkers in the eruption process.
Novel metabolites from Trichoderma atroviride against human prostate cancer cells and their inhibitory effect on Helicobacter pylori and Shigella toxin producing Escherichia coli

Saravanakumar K, Mandava S, Chellia R, Jeevithan E, Babu Yelamanchi RS, Mandava D, et al.

Microb Pathog, 2018 Oct 10;126:19-26.
PMID: 30316006 DOI: 10.1016/j.micpath.2018.10.011

The present study aimed to purify and identify the metabolites from T. atroviride using high-performance liquid chromatography (HPLC) and 1H and 13C nuclear magnetic resonance spectrometer (NMR) followed by analyzing their toxicological, antibacterial and anticancer properties. This work identified two metabolites - TM1 and TM2. TM1 was in two forms: (i) 1, 3-dione-5, 5-dimethylcyclohexane; and, (ii) 2-enone-3hydroxy -5,5-dimethylcylohex, while TM2 was 4H-1,3-dioxin-4-one-2,3,6-trimethyl. These metabolites did not exhibit any irritant or allergic reaction as revealed by HET- CAM test. TM2 significantly inhibited the growth of H. pylori and Shigella toxin producing Escherichia coli (STEC) as evident by in vitro and microscopic observations of bacterial cell death. TM2 also induced the cell death and cytotoxicity, as revealed by cell viability test and western blot analysis. According to microscopic, flow cytometer and western blot analysis, TM2 treated cells displayed higher ROS, cell death, and apoptosis-related protein expression than TM1 and control. This study concluded that TM2 derived from T. atroviride was a potential therapeutic agent for anti-prostate cancer and antibiotic agent against MDR- H. pylori and STEC and it is also recommended to carry out further in vivo animal model experiments with improved stability of the metabolites for future pharmaceutical trails.

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