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  1. Safura Ramli, Evana Kamarudin, Mazura Bahar
    MyJurnal
    After ABO, Rhesus (Rh) is the second most clinically important blood group regarding transfusion
    and pregnancy induced alloimmunisation. RhD DEL is a subtype of variant RhD, which is difficult to
    determine in a routine blood bank, since it expresses an extremely low level of D antigens. Serologically,
    it can only be detected via adsorption-elution test. To date, there have been limited data available on the
    Rhd DEL phenotype in Malaysia. Thus, this study was carried out to detect DEL phenotype among RhD
    negative donors in Malaysia. A total of 43 RhD-negative blood samples were collected from National
    Blood Centre, Malaysia. Rh phenotype for each sample was tested, followed by adsorption elution
    technique. Then, identification of DEL carrying RHD1227A allele was performed via SSP-PCR. Rhphenotype
    identified were ccee (79.07%), Ccee (13.95%), 4.65% of ccEe phenotype and only 2.33% of
    CCee phenotype. One (2.3%) out of the 43 samples was identified as DEL phenotype carrying RHD1227A
    allele when tested using SSP-PCR, but none was identified from adsorption-elution. A larger sample
    size is recommended to determine the exact prevalence of DEL phenotype, as well as specificity and
    sensitivity between SSP-PCR as compared with the traditional adsorption elution technique.
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