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  1. Melati Khalid, Mohamad Aris Mohd Moklas
    MyJurnal
    Aromatic L-amino acid decarboxylase deficiency (AADC) is a rare autosomal recessive pediatric neurotransmitter disease. To date it remains poorly understood mainly due to an absence of a disease model. The dopaminergic neuroblastoma cell SH-SY5Y was chosen to develop our AADC deficiency model. These cells are not native dopamine synthesizers. Objective: To develop a dopamine-producing cellular model of AADC deficiency using SH-SY5Y neuroblastoma cells. Methods: Dopamine pathway proteins were identified with Western Blotting. Dopaminergic differentiation was attempted using all-trans retinoic acid (ATRA) with dopamine detection via HPLC-ECD post alumina extraction. Treatment with L-DOPA provided SH-SY5Y with excess precursor. RT-PCR was used to determine the expression of markers of mature neurons. Results: Western Blot screening identified AADC, dopamine β-hydroxylase and tyrosine hyrdoxylase proteins, indicative of a dopaminergic pathway. ATRA was unsuccessful in producing dopamine from the cells. L-DOPA treatment however, generated dopamine first visible as a HPLC-ECD peak 30 minutes post-incubation. Prior to this, SH-SY5Y dopamine synthesis from L-DOPA has never been documented. This de novo synthesis is then inhibited using benserazide to form our AADC deficiency cell model. RT-PCR showed that SH-SY5Y cells express markers of mature neurons in its ‘native’ state and is not affected by L-DOPA and benserazide treatment. This cell model will potentially benefit many areas of AADC deficiency research. Conclusion: SH-SY5Y cells produced HPLC-ECD measureable amounts of dopamine with the addition of L-DOPA. Our model of AADC deficiency is generated by quelling the dopamine production with Benserazide.
  2. Muhamad Zulhusni Abdul Wahab, Muhammad Zaim Zainal Abidin, Fatin Nadzirah Zakaria, Mohamad Aris Mohd Moklas, Zulkhairi Amom, Nina Keterina Hashim, et al.
    MyJurnal
    Centella asiatica is one of the traditional herbs consumed by many communities due to its wide range of applications such as treating Parkinsonism, promoting memory enhancement, and preventing oxidative stress. This study was conducted to investigate the neuroprotective potential of aqueous C. asiatica extract (CAE) against neurodegeneration induced by chronic stress. Administration of CAE at three different dosages (200 mg/kg/day, 400 mg/ kg/day and 800 mg/kg/day) was conducted for a period of 21 days along with exposure to chronic stress using restrainer and forced swimming regimes. The administration of CAE significantly improved the thickness of dentate gyrus and reduced the amount of neuronal cell death at dentate gyrus and CA3 (p
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