Introduction: Workplace violence (WPV) among healthcare workers (HCW) remains a significant public health issue in Malaysia. This study aimed to determine the prevalence and associated factors of WPV among HCW in Emergency Departments of public hospitals in Melaka. Methods: A cross-sectional study was conducted among 231 HCW who were selected in Emergency Departments (ED) from three public hospitals in Melaka through proportionate stratified random sampling method. Data was collected using self-administered questionnaire which consists of three parts (personal and workplace data, workplace violence, and psychosocial workplace environment factors). The period of workplace violence studied was any occurrence of it within six months prior to this study. The data were anal-ysed using Statistical Package for Social Sciences (SPSS) software version 24. Results: The prevalence of WPV was found to be 38%, of which 88.9% were psychological violence, and were mostly perpetrated by combinations of type of perpetrators (51.9%). Multiple logistic regression shows that those aged 40 years and below have 5.4 times more likely to experience workplace violence compared to other age group, respondents with low job support has 2.9 times more likely to experience workplace violence compared to its counterpart, and respondents who work in secondary hospital have 2.7 times more likely to experience workplace violence compared to those who work in tertiary hospital. Conclusion: Preventive measures to prevent this issue should be in placed to tackle WPV especially among the at risk groups.