In this work, the bioactive compounds which was obtained by extracting Quercus infectoria via two extraction methods; Soxhlet and supercritical carbon dioxide (SC-CO2) extraction, were analyzed using total phenolic content and DPPH (2,2-diphenyl-1-picryl hydrazyl) free radical scavenging activity analysis. The aim of this study is to compare the total phenolic content and antioxidant activity of Quercus infectoria extract acquired from SC-CO2 extraction with those from Soxhlet extraction method. The results showed the used of SC-CO2 extraction give the lowest extraction yield as compared to Soxhlet extraction. The selectivity of Q. infectoria extracts using SC-CO2 extraction was better which in contrast with Soxhlet extraction method since it shows higher total phenolic content (143.75 ± 1.06 mg GAE/g sample). This study also revealed that the extracts from both extraction methods can posses’ antioxidant activity and comparable to those obtained from commercial antioxidant.